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2.A.52.1.4
High affinity Ni2+ uptake porter, NixA.  Transcription is regulated by NixR (Wolfram et al. 2006).

Accession Number:Q48262
Protein Name:NixA aka HP1077
Length:331
Molecular Weight:37034.00
Species:Helicobacter pylori (Campylobacter pylori) [210]
Number of TMSs:7
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Ni2+

Cross database links:

HEGENOM: HBG322888
DIP: DIP-3647N
RefSeq: NP_207868.1   
Entrez Gene ID: 899613   
Pfam: PF03824   
KEGG: hpy:HP1077   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0046872 F:metal ion binding
GO:0015099 F:nickel ion transmembrane transporter activity
GO:0015675 P:nickel ion transport
GO:0055085 P:transmembrane transport

References (3)

[1] “Helicobacter pylori nickel-transport gene nixA: synthesis of catalytically active urease in Escherichia coli independent of growth conditions.”  Mobley H.L.T.et.al.   7651142
[2] “The complete genome sequence of the gastric pathogen Helicobacter pylori.”  Tomb J.-F.et.al.   9252185
[3] “Membrane topology of the NixA nickel transporter of Helicobacter pylori: two nickel transport-specific motifs within transmembrane helices II and III.”  Fulkerson J.F. Jr.et.al.   10692379

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKLWFPYFLA IVFLHALGLA LLFMANNASF YAAASMAYML GAKHAFDADH IACIDNTIRK 
61:	LTQQGKNAYG VGFYFSMGHS SVVILMTIIS AFAIAWAKEH TPMLEEIGGV VGTLVSGLFL 
121:	LIIGLLNAII LLDLLKIFKK SHSNESLSQQ QNEEIERLLT SRGLLNRFFK PLFNFVSKSW 
181:	HIYPIGFLFG LGFDTASEIA LLALSSSAIK VSMVGMLSLP ILFAAGMSLF DTLDGAFMLK 
241:	AYDWAFKTPL RKIYYNISIT ALSVFIALFI GLIELFQVVS EKLHLKFENR LLRALQSLEF 
301:	TDLGYYLVGL FVIAFLGSFF LWKIKFSKLE S