TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.53.2.19
Prestin (Solute carrier family 26 member 5).  The motor protein responsible for the somatic electromotility of cochlear outer hair cells; essential for normal hearing sensitivity and frequency selectivity of mammals. Prestin transports a wide variety of monovalent and divalent anions. Many SulP transporters in humans are involved in genetic diseases.They have C-terminal hydrophilic STAS domains that are essential for plasma membrane targeting and protein function. The crystal structure of this STAS domain has been solved at 1.57 A resolution (Pasqualetto et al. 2010).  It senses voltage and binds anions for induction of conformational changes (He et al. 2013).  Prestin's 7+7 inverted repeat architecture suggests a central cavity as the substrate-binding site located midway within the anion permeation pathway. Anion binding to this site controls the electromotile activity of prestin (Gorbunov et al. 2014).

Accession Number:P58743
Protein Name:Prestin
Length:744
Molecular Weight:81264.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate anions

Cross database links:

Genevestigator: P58743
eggNOG: COG0659
HEGENOM: HOG000006546
Entrez Gene ID: 375611   
Pfam: PF01740    PF00916   
KEGG: hsa:375611   

Gene Ontology

GO:0016323 C:basolateral plasma membrane
GO:0016021 C:integral to membrane
GO:0016328 C:lateral plasma membrane
GO:0008271 F:secondary active sulfate transmembrane transporter activity
GO:0008360 P:regulation of cell shape
GO:0042391 P:regulation of membrane potential
GO:0007605 P:sensory perception of sound

References (3)

[1] “Prestin, a cochlear motor protein, is defective in non-syndromic hearing loss.”  Liu X.Z.et.al.   12719379
[2] “The DNA sequence of human chromosome 7.”  Hillier L.W.et.al.   12853948
[3] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MDHAEENEIL AATQRYYVER PIFSHPVLQE RLHTKDKVPD SIADKLKQAF TCTPKKIRNI 
61:	IYMFLPITKW LPAYKFKEYV LGDLVSGIST GVLQLPQGLA FAMLAAVPPI FGLYSSFYPV 
121:	IMYCFLGTSR HISIGPFAVI SLMIGGVAVR LVPDDIVIPG GVNATNGTEA RDALRVKVAM 
181:	SVTLLSGIIQ FCLGVCRFGF VAIYLTEPLV RGFTTAAAVH VFTSMLKYLF GVKTKRYSGI 
241:	FSVVYSTVAV LQNVKNLNVC SLGVGLMVFG LLLGGKEFNE RFKEKLPAPI PLEFFAVVMG 
301:	TGISAGFNLK ESYNVDVVGT LPLGLLPPAN PDTSLFHLVY VDAIAIAIVG FSVTISMAKT 
361:	LANKHGYQVD GNQELIALGL CNSIGSLFQT FSISCSLSRS LVQEGTGGKT QLAGCLASLM 
421:	ILLVILATGF LFESLPQAVL SAIVIVNLKG MFMQFSDLPF FWRTSKIELT IWLTTFVSSL 
481:	FLGLDYGLIT AVIIALLTVI YRTQSPSYKV LGKLPETDVY IDIDAYEEVK EIPGIKIFQI 
541:	NAPIYYANSD LYSNALKRKT GVNPAVIMGA RRKAMRKYAK EVGNANMANA TVVKADAEVD 
601:	GEDATKPEEE DGEVKYPPIV IKSTFPEEMQ RFMPPGDNVH TVILDFTQVN FIDSVGVKTL 
661:	AGIVKEYGDV GIYVYLAGCS AQVVNDLTRN RFFENPALWE LLFHSIHDAV LGSQLREALA 
721:	EQEASAPPSQ EDLEPNATPA TPEA