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2.A.55.1.1
High-affinity Me2+ (Fe2+, Mn2+, Zn2+, Cu2+, Cd2+, Ni2+, Co2+) uptake transporter, Smf1p or ESP1p of 575 aas and 11 TMSs.  Important for oxidative stress protection. Its activity is regulated by the Tre1 protein alone, and it's degradation is dependent on the Bsd2, Rsp5, Tre1 and Tre2 proteins (Stimpson et al. 2006).

Accession Number:P38925
Protein Name:SMF1 aka Smf1p aka ESP1 aka YOL122C
Length:575
Molecular Weight:63264.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Fe2+, Mn2+, Zn2+, Cu2+, Cd2+, Ni2+, Co2+

Cross database links:

Genevestigator: P38925
eggNOG: fuNOG07564
HEGENOM: HBG312498
RefSeq: NP_014519.1   
Entrez Gene ID: 854027   
Pfam: PF01566   
KEGG: sce:YOL122C   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015082 F:di-, tri-valent inorganic cation transmembr...
GO:0046872 F:metal ion binding
GO:0015295 F:solute:hydrogen symporter activity
GO:0015691 P:cadmium ion transport
GO:0006876 P:cellular cadmium ion homeostasis
GO:0006878 P:cellular copper ion homeostasis
GO:0030026 P:cellular manganese ion homeostasis
GO:0006825 P:copper ion transport
GO:0006826 P:iron ion transport
GO:0006828 P:manganese ion transport

References (8)

[1] “Two related genes encoding extremely hydrophobic proteins suppress a lethal mutation in the yeast mitochondrial processing enhancing protein.”  West A.H.et.al.   1447206
[2] “A yeast manganese transporter related to the macrophage protein involved in conferring resistance to mycobacteria.”  Supek F.et.al.   8643535
[3] “DNA sequence analysis of a 10 624 bp fragment of the left arm of chromosome XV from Saccharomyces cerevisiae reveals a RNA binding protein, a mitochondrial protein, two ribosomal proteins and two new open reading frames.”  Lafuente M.J.et.al.   8896268
[4] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.”  Dujon B.et.al.   9169874
[5] “The family of SMF metal ion transporters in yeast cells.”  Cohen A.et.al.   10930410
[6] “Saccharomyces cerevisiae expresses three functionally distinct homologues of the nramp family of metal transporters.”  Portnoy M.E.et.al.   11027260
[7] “Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.”  Gruhler A.et.al.   15665377
[8] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MVNVGPSHAA VAVDASEARK RNISEEVFEL RDKKDSTVVI EGEAPVRTFT SSSSNHERED 
61:	TYVSKRQVMR DIFAKYLKFI GPGLMVSVAY IDPGNYSTAV DAGASNQFSL LCIILLSNFI 
121:	AIFLQCLCIK LGSVTGLDLS RACREYLPRW LNWTLYFFAE CAVIATDIAE VIGTAIALNI 
181:	LIKVPLPAGV AITVVDVFLI MFTYKPGASS IRFIRIFECF VAVLVVGVCI CFAIELAYIP 
241:	KSTSVKQVFR GFVPSAQMFD HNGIYTAISI LGATVMPHSL FLGSALVQPR LLDYDVKHGN 
301:	YTVSEEQDKV KKSKSTEEIM EEKYFNYRPT NAAIKYCMKY SMVELSITLF TLALFVNCAI 
361:	LVVAGSTLYN SPEADGADLF TIHELLSRNL APAAGTIFML ALLLSGQSAG VVCTMSGQIV 
421:	SEGHINWKLQ PWQRRLATRC ISIIPCLVIS ICIGREALSK ALNASQVVLS IVLPFLVAPL 
481:	IFFTCKKSIM KTEITVDHTE EDSHNHQNNN DRSAGSVIEQ DGSSGMEIEN GKDVKIVYMA 
541:	NNWIITVIAI IVWLFLSLLN VYAIVQLGMS HGDIS