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2.A.55.2.2
Me2+ (Fe2+, Cd2+, Co2+):H+ symporter, DCT1 (Nramp2) (Splice variant isoforms serve different functions). The stoichiometry between metal ion and proton in the symport process catalyzed by DCT1 varies under different conditions due to mechanistic proton slip. A single reciprocal mutation, I144F, in TMS2 of DCT1 abolished the metal ion transport activity, increased the slip currents, and generated sodium slip currents (Nevo, 2007). A double mutation, adding F227I in TMS4 to I144F restored the uptake activity of DCT1 and reduced the slip currents. Thus, these regions are important in coupling metal ion and proton symport (Nevo, 2007).  NRAMP2 also serves as the Sindbis alpha virus receptor (Rose et al. 2011).  Three isoforms are expressed differentially in different cell types and are developmentally regulated (Ding et al. 2013).

Accession Number:O54902
Protein Name:NRM2 aka DCT1 aka SLC11A2 aka NRAMP2
Length:568
Molecular Weight:62277.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Fe2+, Cd2+, Co2+, H+

Cross database links:

Genevestigator: O54902
eggNOG: maNOG05468
RefSeq: NP_037305.1   
Entrez Gene ID: 25715   
Pfam: PF01566   
KEGG: rno:25715   

Gene Ontology

GO:0045177 C:apical part of cell
GO:0016021 C:integral to membrane
GO:0031902 C:late endosome membrane
GO:0005765 C:lysosomal membrane
GO:0046870 F:cadmium ion binding
GO:0015086 F:cadmium ion transmembrane transporter activity
GO:0050897 F:cobalt ion binding
GO:0015087 F:cobalt ion transmembrane transporter activity
GO:0005507 F:copper ion binding
GO:0005375 F:copper ion transmembrane transporter activity
GO:0015093 F:ferrous iron transmembrane transporter acti...
GO:0005506 F:iron ion binding
GO:0015094 F:lead ion transmembrane transporter activity
GO:0030145 F:manganese ion binding
GO:0005384 F:manganese ion transmembrane transporter act...
GO:0016151 F:nickel ion binding
GO:0015099 F:nickel ion transmembrane transporter activity
GO:0008270 F:zinc ion binding
GO:0015684 P:ferrous iron transport
GO:0046686 P:response to cadmium ion
GO:0010039 P:response to iron ion
GO:0010288 P:response to lead ion
GO:0010042 P:response to manganese ion

References (2)

[1] “Cloning and characterization of a mammalian proton-coupled metal-ion transporter.”  Gunshin H.et.al.   9242408
[2] “Nramp2 is mutated in the anemic Belgrade (b) rat: evidence of a role for Nramp2 in endosomal iron transport.”  Fleming M.D.et.al.   9448300

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MVLDPEEKIP DDGASGDHGD SASLGAINPA YSNSSLPHST GDSEEPFTTY FDEKIPIPEE 
61:	EYSCFSFRKL WAFTGPGFLM SIAYLDPGNI ESDLQSGAVA GFKLLWVLLL ATIVGLLLQR 
121:	LAARLGVVTG LHLAEVCHRQ YPKVPRIILW LMVELAIIGS DMQEVIGSAI AINLLSAGRV 
181:	PLYGGVLITI ADTFVFLFLD KYGLRKLEAF FGFLITIMAL TFGYEYVTVK PSQSQVLRGM 
241:	FVPSCSGCHT PQVEQAVGIV GAVIMPHNMY LHSALVKSRQ VNRANKQEVR EANKYFFIES 
301:	CIALFVSFII NVFVVSVFAE AFFEKTNEQV VEVCRNSSSP HADLFPNDNS TLAVDIYKGG 
361:	VVLGCYFGPA ALYIWAVGIL AAGQSSTMTG TYSGQFVMEG FLNLKWSRFA RVILTRSIAI 
421:	IPTLLVAVFQ DVEHLTGMND FLNVLQSLQL PFALIPILTF TSLRPVMSEF SNGIGWRIAG 
481:	GILVLLVCSI NMYFVVVYVQ ELGHVALYVV AAVVSVAYLG FVFYLGWQCL IALGLSFLDC 
541:	GRSYHLGLTA RPEIYLLNTV DAVSLVSR