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2.A.55.3.1
Me2+ (Mn2+, Fe2+, Cd2+, Co2+, Zn2+, Ni2+):H+ symporter, MntH (Mn2+ · MntR and Fe2+ · Fur repressible). Specific resides in TMS1 and 6 line the pore and play a role in pH regulation (Courville et al., 2004; Haemig et al. 2010). Important for virulence in Salmonella (Haemig et al. 2010). Important for virulence in Salmonella (Karlinsey et al., 2010).

Accession Number:P0A769
Protein Name:Manganese transport protein MntH aka YfeP aka B2392
Length:412
Molecular Weight:44194.00
Species:Escherichia coli [83333]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Mn2+, Fe2+, Cd2+, Co2+, Zn2+, Ni2+

Cross database links:

Genevestigator: P0A769
EchoBASE: EB3909
EcoGene: EG14157
eggNOG: COG1914
HEGENOM: HBG312498
DIP: DIP-48004N
RefSeq: AP_002991.1    NP_416893.1   
Entrez Gene ID: 946899   
Pfam: PF01566   
BioCyc: EcoCyc:YFEP-MONOMER    ECOL168927:B2392-MONOMER   
KEGG: ecj:JW2388    eco:b2392   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015086 F:cadmium ion transmembrane transporter activity
GO:0015078 F:hydrogen ion transmembrane transporter acti...
GO:0030145 F:manganese ion binding
GO:0005384 F:manganese ion transmembrane transporter act...
GO:0015293 F:symporter activity
GO:0015292 F:uniporter activity
GO:0015691 P:cadmium ion transport
GO:0034599 P:cellular response to oxidative stress
GO:0006824 P:cobalt ion transport
GO:0015684 P:ferrous iron transport
GO:0006828 P:manganese ion transport

References (8)

[1] “The NRAMP proteins of Salmonella typhimurium and Escherichia coli are selective manganese transporters involved in the response to reactive oxygen.”  Kehres D.G.et.al.   10844693
[2] “Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.”  Yamamoto Y.et.al.   9205837
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Identification of the Escherichia coli K-12 Nramp orthologue (MntH) as a selective divalent metal ion transporter.”  Makui H.et.al.   10712688
[6] “Determination of transmembrane topology of the Escherichia coli natural resistance-associated macrophage protein (Nramp) ortholog.”  Courville P.et.al.   14607838
[7] “Importance of conserved acidic residues in mntH, the Nramp homolog of Escherichia coli.”  Haemig H.A.et.al.   15630547
[8] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MTNYRVESSS GRAARKMRLA LMGPAFIAAI GYIDPGNFAT NIQAGASFGY QLLWVVVWAN 
61:	LMAMLIQILS AKLGIATGKN LAEQIRDHYP RPVVWFYWVQ AEIIAMATDL AEFIGAAIGF 
121:	KLILGVSLLQ GAVLTGIATF LILMLQRRGQ KPLEKVIGGL LLFVAAAYIV ELIFSQPNLA 
181:	QLGKGMVIPS LPTSEAVFLA AGVLGATIMP HVIYLHSSLT QHLHGGSRQQ RYSATKWDVA 
241:	IAMTIAGFVN LAMMATAAAA FHFSGHTGVA DLDEAYLTLQ PLLSHAAATV FGLSLVAAGL 
301:	SSTVVGTLAG QVVMQGFIRF HIPLWVRRTV TMLPSFIVIL MGLDPTRILV MSQVLLSFGI 
361:	ALALVPLLIF TSDSKLMGDL VNSKRVKQTG WVIVVLVVAL NIWLLVGTAL GL