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2.A.57.1.3
Equilibrative high affinity nucleoside transporter (nitrobenzyl-thioinosine-sensitive) (transports thymidine, adenosine, cytosine, and guanosine; inosine and hypoxanthine are poorly transported).  Uridine uptake in the basolateral membrane of sertoli cells is selectively inhibited by 100 nM nitrobenzylmercaptopurine riboside (NBMPR, 6-S-[(4-nitrophenyl)methyl]-6-thioinosine) (Klein et al. 2013).

Accession Number:O54698
Protein Name:ENT1 aka rENT1 aka SLC29A1
Length:457
Molecular Weight:50017.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Thymidine, Adenosine, Cytosine, Guanosine, Inosine, Hypoxanthine, Uridine

Cross database links:

Genevestigator: O54698
eggNOG: roNOG05802
RefSeq: NP_113872.1   
Entrez Gene ID: 63997   
Pfam: PF01733   
KEGG: rno:63997   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005337 F:nucleoside transmembrane transporter activity
GO:0071333 P:cellular response to glucose stimulus
GO:0071456 P:cellular response to hypoxia
GO:0007595 P:lactation
GO:0060079 P:regulation of excitatory postsynaptic membr...
GO:0030431 P:sleep
GO:0015862 P:uridine transport

References (2)

[1] “Molecular cloning and functional characterization of nitrobenzylthioinosine (NBMPR)-sensitive (es) and NBMPR-insensitive (ei) equilibrative nucleoside transporter proteins (rENT1 and rENT2) from rat tissues.”  Yao S.Y.M.et.al.   9353301
[2] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334

External Searches:

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  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MTTSHQPQDR YKAVWLIFFV LGLGTLLPWN FFITATQYFT SRLNTSQNIS LVTNQSCEST 
61:	EALADPSVSL PARSSLSAIF NNVMTLCAML PLLIFTCLNS FLHQKVSQSL RILGSLLAIL 
121:	LVFLVTATLV KVQMDALSFF IITMIKIVLI NSFGAILQAS LFGLAGVLPA NYTAPIMSGQ 
181:	GLAGFFTSVA MICAVASGSK LSESAFGYFI TACAVVILAI LCYLALPWME FYRHYLQLNL 
241:	AGPAEQETKL DLISEGEEPR GGREESGVPG PNSLPANRNQ SIKAILKSIW VLALSVCFIF 
301:	TVTIGLFPAV TAEVESSIAG TSPWKNCYFI PVACFLNFNV FDWLGRSLTA ICMWPGQDSR 
361:	WLPVLVACRV VFIPLLMLCN VKQHHYLPSL FKHDVWFITF MAAFAFSNGY LASLCMCFGP 
421:	KKVKPAEAET AGNIMSFFLC LGLALGAVLS FLLRALV