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2.A.57.1.8
Equilibrative nucleoside transporter 2 (36 kDa nucleolar protein HNP36) (Delayed-early response protein 12) (Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter) (Equilibrative NBMPR-insensitive nucleoside transporter) (Hydrophobic nucleolar protein, 36 kDa) (Nucleoside transporter, ei-type) (Solute carrier family 29 member 2). Takes up the adenosine analogue, fludarabine (AraFA), used to treat cancer (lymphomas and leukemia) (Gorzkiewicz et al. 2018).

Accession Number:Q14542
Protein Name:Equilibrative nucleoside transporter 2
Length:456
Molecular Weight:50113.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Basolateral cell membrane1 / Multi-pass membrane protein2
Substrate Nucleosides

Cross database links:

Genevestigator: Q14542
eggNOG: NOG249415
HEGENOM: HOG000007684
Entrez Gene ID: 3177   
Pfam: PF01733   
KEGG: hsa:3177   

Gene Ontology

GO:0016323 C:basolateral plasma membrane
GO:0005887 C:integral to plasma membrane
GO:0031965 C:nuclear membrane
GO:0005730 C:nucleolus
GO:0005337 F:nucleoside transmembrane transporter activity
GO:0015853 P:adenine transport
GO:0008283 P:cell proliferation
GO:0032869 P:cellular response to insulin stimulus
GO:0015854 P:guanine transport
GO:0035344 P:hypoxanthine transport
GO:0007595 P:lactation
GO:0006139 P:nucleobase-containing compound metabolic process
GO:0035364 P:thymine transport
GO:0015862 P:uridine transport

References (10)

[1] “A mammalian delayed-early response gene encodes HNP36, a novel, conserved nucleolar protein.”  Williams J.B.et.al.   7639753
[2] “Molecular cloning and characterization of a nitrobenzylthioinosine-insensitive (ei) equilibrative nucleoside transporter from human placenta.”  Griffiths M.et.al.   9396714
[3] “Cloning of the human equilibrative, nitrobenzylmercaptopurine riboside (NBMPR)-insensitive nucleoside transporter ei by functional expression in a transport-deficient cell line.”  Crawford C.R.et.al.   9478986
[4] “Localization of human equilibrative nucleoside transporters, hENT1 and hENT2, in renal epithelial cells.”  Mangravite L.M.et.al.   12527552
[5] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[6] “Human chromosome 11 DNA sequence and analysis including novel gene identification.”  Taylor T.D.et.al.   16554811
[7] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[8] “Functional analysis of site-directed glycosylation mutants of the human equilibrative nucleoside transporter-2.”  Ward J.L.et.al.   12590919
[9] “Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.”  Gauci S.et.al.   19413330
[10] “Functional characterization and haplotype analysis of polymorphisms in the human equilibrative nucleoside transporter, ENT2.”  Owen R.P.et.al.   16214850

External Searches:

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  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MARGDAPRDS YHLVGISFFI LGLGTLLPWN FFITAIPYFQ ARLAGAGNST ARILSTNHTG 
61:	PEDAFNFNNW VTLLSQLPLL LFTLLNSFLY QCVPETVRIL GSLLAILLLF ALTAALVKVD 
121:	MSPGPFFSIT MASVCFINSF SAVLQGSLFG QLGTMPSTYS TLFLSGQGLA GIFAALAMLL 
181:	SMASGVDAET SALGYFITPC VGILMSIVCY LSLPHLKFAR YYLANKSSQA QAQELETKAE 
241:	LLQSDENGIP SSPQKVALTL DLDLEKEPES EPDEPQKPGK PSVFTVFQKI WLTALCLVLV 
301:	FTVTLSVFPA ITAMVTSSTS PGKWSQFFNP ICCFLLFNIM DWLGRSLTSY FLWPDEDSRL 
361:	LPLLVCLRFL FVPLFMLCHV PQRSRLPILF PQDAYFITFM LLFAVSNGYL VSLTMCLAPR 
421:	QVLPHEREVA GALMTFFLAL GLSCGASLSF LFKALL