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2.A.6.2.13
Multidrug/dye/detergent resistance protein, YhiU/YhiV or MdtE/MdtF (Nishino and Yamaguchi 2001) MdtE (YhiU) is listed under TC# 8.A.1.6.3.  The system exports erythromycin, doxorubicin, crystal violet, ethidium, rhodamine 6G, TPP, benzalkonium, SDS, deoxycholate and growth inhibitory steroid hormones (estradiol and progesterone) (Elkins and Mullis, 2006).

Accession Number:P37637
Protein Name:YhiV aka B3514
Length:1037
Molecular Weight:111517.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate erythromycin, doxorubicin, Crystal violet, ethidium, Rhodamine 6G, TPP, Benzalkonium, SDS, Deoxycholate, estradiol, progesterone

Cross database links:

Genevestigator: P37637
EchoBASE: EB2152
EcoGene: EG12241
eggNOG: COG0841
HEGENOM: HBG293864
RefSeq: AP_004279.1    NP_417971.1   
Entrez Gene ID: 948030   
Pfam: PF00873   
BioCyc: EcoCyc:YHIV-MONOMER    ECOL168927:B3514-MONOMER   
KEGG: ecj:JW3482    eco:b3514   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity
GO:0046677 P:response to antibiotic
GO:0006810 P:transport

References (10)

[1] “Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes.”  Sofia H.J.et.al.   8041620
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Analysis of a complete library of putative drug transporter genes in Escherichia coli.”  Nishino K.et.al.   11566977
[5] “EvgA of the two-component signal transduction system modulates production of the yhiUV multidrug transporter in Escherichia coli.”  Nishino K.et.al.   11914367
[6] “Escherichia coli gene expression responsive to levels of the response regulator EvgA.”  Masuda N.et.al.   12399493
[7] “Transcriptional regulation of drug efflux genes by EvgAS, a two-component system in Escherichia coli.”  Eguchi Y.et.al.   14523115
[8] “Regulatory network of acid resistance genes in Escherichia coli.”  Masuda N.et.al.   12694615
[9] “Protein complexes of the Escherichia coli cell envelope.”  Stenberg F.et.al.   16079137
[10] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MANYFIDRPV FAWVLAIIMM LAGGLAIMNL PVAQYPQIAP PTITVSATYP GADAQTVEDS 
61:	VTQVIEQNMN GLDGLMYMSS TSDAAGNASI TLTFETGTSP DIAQVQVQNK LQLAMPSLPE 
121:	AVQQQGISVD KSSSNILMVA AFISDNGSLN QYDIADYVAS NIKDPLSRTA GVGSVQLFGS 
181:	EYAMRIWLDP QKLNKYNLVP SDVISQIKVQ NNQISGGQLG GMPQAADQQL NASIIVQTRL 
241:	QTPEEFGKIL LKVQQDGSQV LLRDVARVEL GAEDYSTVAR YNGKPAAGIA IKLAAGANAL 
301:	DTSRAVKEEL NRLSAYFPAS LKTVYPYDTT PFIEISIQEV FKTLVEAIIL VFLVMYLFLQ 
361:	NFRATIIPTI AVPVVILGTF AILSAVGFTI NTLTMFGMVL AIGLLVDDAI VVVENVERVI 
421:	AEDKLPPKEA THKSMGQIQR ALVGIAVVLS AVFMPMAFMS GATGEIYRQF SITLISSMLL 
481:	SVFVAMSLTP ALCATILKAA PEGGHKPNAL FARFNTLFEK STQHYTDSTR SLLRCTGRYM 
541:	VVYLLICAGM AVLFLRTPTS FLPEEDQGVF MTTAQLPSGA TMVNTTKVLQ QVTDYYLTKE 
601:	KDNVQSVFTV GGFGFSGQGQ NNGLAFISLK PWSERVGEEN SVTAIIQRAM IALSSINKAV 
661:	VFPFNLPAVA ELGTASGFDM ELLDNGNLGH EKLTQARNEL LSLAAQSPNQ VTGVRPNGLE 
721:	DTPMFKVNVN AAKAEAMGVA LSDINQTIST AFGSSYVNDF LNQGRVKKVY VQAGTPFRML 
781:	PDNINQWYVR NASGTMAPLS AYSSTEWTYG SPRLERYNGI PSMEILGEAA AGKSTGDAMK 
841:	FMADLVAKLP AGVGYSWTGL SYQEALSSNQ APALYAISLV VVFLALAALY ESWSIPFSVM 
901:	LVVPLGVVGA LLATDLRGLS NDVYFQVGLL TTIGLSAKNA ILIVEFAVEM MQKEGKTPIE 
961:	AIIEAARMRL RPILMTSLAF ILGVLPLVIS HGAGSGAQNA VGTGVMGGMF AATVLAIYFV 
1021:	PVFFVVVEHL FARFKKA