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2.A.6.3.1
Putative lipooligosaccharide nodulation factor exporter, NolG (1065 aas; previously thought to be 3 ORFs, NolGHI, an artifact due to sequencing errors and consequent frameshifting (Baev et al. 1991; Ardourel et al. 1994).

Accession Number:P25197
Protein Name:Nodulation protein nolG
Length:1065
Molecular Weight:114428.00
Species:Rhizobium meliloti [382]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Peripheral membrane protein2 / Cytoplasmic side3
Substrate LPS nodulation factor

Cross database links:

HEGENOM: HBG293864
RefSeq: NP_435726.1   
Entrez Gene ID: 1235516   
Pfam: PF00873   
BioCyc: SMEL266834:SMA0875-MONOMER   
KEGG: sme:SMa0875   

Gene Ontology

GO:0016020 C:membrane
GO:0005215 F:transporter activity
GO:0009877 P:nodulation
GO:0006810 P:transport

References (2)

[1] “Six nodulation genes of nod box locus 4 in Rhizobium meliloti are involved in nodulation signal production: nodM codes for D-glucosamine synthetase.”  Baev N.et.al.   1909418
[2] “Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid.”  Barnett M.J.et.al.   11481432

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MFLTRISINH PVFATMMMVM ILVLGLFSYG RLGVDHYPET DLPVVVVATT YTGASPESVE 
61:	SEISRPIEAA LNTIGGIDTI TSESYEGRSI VVVQFEVDVD SQDAAQEVRD RVARLETKFP 
121:	DGVATPQVTR YKPEGQAILS VAVSSTSRTL PEITTLATRV INNRLSVISG VGQVSLIGSS 
181:	ERQVLVVVDP DRLGAYGLAV STVIEAIRGE NQDRAAGTLI SGINQRIVTV EGRIANTSGF 
241:	NRIIVAQRNG YPVYLSEVAT ILDTGAEVTS LANYQGQTTL GLHIVKVQGA NTVEVASAVR 
301:	REVSALNAEL TKDNVQLTIT RDNSRPIASQ VSQVQRTLVE GGVLSVLIVF IFLNSWRSTV 
361:	ITGLTLPISV IGTFAAIYAL GFTLNIMTLM ALSLSIGILI DDAIVVRENI TRHLQMGKDP 
421:	VRAALDGTNE IGLAVLSTTL CIVAVFLPVA FMGGLIGRFF LQFGVTVAVA VVISLFVSFT 
481:	LDPMLSSVWC DPQSQKTAKR GFFGQLIERF DQWFEGLASR YRSVIYFTFD YRKTTIAIVL 
541:	GMFVVSLLLV PRIGTEFLPP PDQGEVSISL EANEGASLDY MAAKVGQIER ALREFNYVSS 
601:	TYSTINSGEM RGFNKALVAV QLVHSSQRRL KTAETLGPIR RRLSRIAGLE ISVGQRSEVV 
661:	GSIKPLQLSI LGDGDEELRR ISDHITSVLA AIPGATEIES SIEKLRPTLA VRVRREAASD 
721:	LGVSIATIGD TLRSLVAGDA ISVWNSPDGE THDVVVRLPA AGRENAAQLR NLPIATARMD 
781:	DNGKPIMVLL DQVADVVEST APAQITRKDL SRDIRISSNI EGRTLGDVVA DLKAAMTKMD 
841:	IPVGFRISFG GDAENLTEST AYALQSLAMA VIFIYIILAS QFGSFIQPIA IIMTMPLSLM 
901:	GVLLGLLFTG STLNMFSMIG IMMLMGLVTK NAILLVDYSN LGVREGKSLR QSLADAGAVR 
961:	LRPIVMTTLA MIFGMLPTAL GLGEGGAQRA PMAHAIIGGL ISSTLLSLVF VPVVLTYLDA 
1021:	FAGRVRRWVP SPTGSNASAQ HDGSDKTKTP ACALTSQDQL GTTIM