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2.A.66.1.13
Drug (monovalent and divalent biocides; fluoroquinolones including norfloxacin and ciprofloxacin) efflux pump, SvrA (MepA) (Kaatz et al., 2006). Also exports tigecycline (McAleese et al., 2005).

Accession Number:Q2G140
Protein Name:Multidrug export protein mepA
Length:451
Molecular Weight:48776.00
Species:Staphylococcus aureus (strain NCTC 8325) [93061]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Fluoroquinolones, Tigecycline

Cross database links:

eggNOG: COG0534
HEGENOM: HBG504134
RefSeq: YP_498905.1   
Entrez Gene ID: 3919542   
Pfam: PF01554   
BioCyc: SAUR93061:SAOUHSC_00315-MONOMER   
KEGG: sao:SAOUHSC_003   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015297 F:antiporter activity
GO:0015238 F:drug transmembrane transporter activity
GO:0006855 P:drug transmembrane transport
GO:0046677 P:response to antibiotic

References (3)

[1] “Multidrug resistance in Staphylococcus aureus due to overexpression of a novel multidrug and toxin extrusion (MATE) transport protein.”  Kaatz G.W.et.al.   15855507
[2] “Staphylococcus aureus svrA: a gene required for virulence and expression of the agr locus.”  Garvis S.et.al.   12368457
[3] “MepR, a repressor of the Staphylococcus aureus MATE family multidrug efflux pump MepA, is a substrate-responsive regulatory protein.”  Kaatz G.W.et.al.   16569840

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKDEQLYYFE KSPVFKAMMH FSLPMMIGTL LSVIYGILNI YFIGFLEDSH MISAISLTLP 
61:	VFAILMGLGN LFGVGAGTYI SRLLGAKDYS KSKFVSSFSI YGGIALGLIV ILVTLPFSDQ 
121:	IAAILGARGE TLALTSNYLK VMFLSAPFVI LFFILEQFAR AIGAPMVSMI GMLASVGLNI 
181:	ILDPILIFGF DLNVVGAALG TAISNVAAAL FFIIYFMKNS DVVSVNIKLA KPNKEMLSEI 
241:	FKIGIPAFLM SILMGFTGLV LNLFLAHYGN FAIASYGISF RLVQFPELII MGLCEGVVPL 
301:	IAYNFMANKG RMKDVIKAVI MSIGVIFVVC MSAVFTIGHH MVGLFTTDQA IVEMATFILK 
361:	VTMASLLLNG IGFLFTGMLQ ATGQGRGATI MAILQGAIII PVLFIMNALF GLTGVIWSLL 
421:	IAESLCALAA MLIVYLLRDR LTVDTSELIE G