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2.A.66.1.3
Multidrug-resistance efflux pump, NorM (MdtK, NorE or YdhE) (Nishino and Yamaguchi 2001). Exports chloramphenicol, norfloxacin, enoxacin, phosphomycin, doxorubicin, trimethoprim, ethidium, deoxycholate, etc (Long et al., 2008). May also export signals for cell-cell communication (Yang et al., 2006).

Accession Number:P37340
Protein Name:NorM aka YdhE aka B1663
Length:457
Molecular Weight:49447.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Chloramphenicol, norfloxacin, enoxacin, Phosphomycin, doxorubicin, Trimethoprim, ethidium, Deoxycholate

Cross database links:

Genevestigator: P37340
EchoBASE: EB2300
EcoGene: EG12400
eggNOG: COG0534
HEGENOM: HBG479615
RefSeq: AP_002285.1    YP_025307.1   
Entrez Gene ID: 945883   
Pfam: PF01554   
BioCyc: EcoCyc:YDHE-MONOMER   
KEGG: ecj:JW1655    eco:b1663   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015297 F:antiporter activity
GO:0015559 ?:?
GO:0006855 P:drug transmembrane transport
GO:0046677 P:response to antibiotic
GO:0006814 P:sodium ion transport

References (10)

[1] “Analysis of the boundaries of Salmonella pathogenicity island 2 and the corresponding chromosomal region of Escherichia coli K-12.”  Hensel M.et.al.   9023191
[2] “A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.”  Aiba H.et.al.   9097039
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005.”  Riley M.et.al.   16397293
[5] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[6] “NorM, a putative multidrug efflux protein, of Vibrio parahaemolyticus and its homolog in Escherichia coli.”  Morita Y.et.al.   9661020
[7] “The multidrug efflux protein NorM is a prototype of a new family of transporters.”  Brown M.H.et.al.   9987140
[8] “Analysis of a complete library of putative drug transporter genes in Escherichia coli.”  Nishino K.et.al.   11566977
[9] “Relative contributions of the AcrAB, MdfA and NorE efflux pumps to quinolone resistance in Escherichia coli.”  Yang S.et.al.   12615854
[10] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MQKYISEARL LLALAIPVIL AQIAQTAMGF VDTVMAGGYS ATDMAAVAIG TSIWLPAILF 
61:	GHGLLLALTP VIAQLNGSGR RERIAHQVRQ GFWLAGFVSV LIMLVLWNAG YIIRSMENID 
121:	PALADKAVGY LRALLWGAPG YLFFQVARNQ CEGLAKTKPG MVMGFIGLLV NIPVNYIFIY 
181:	GHFGMPELGG VGCGVATAAV YWVMFLAMVS YIKRARSMRD IRNEKGTAKP DPAVMKRLIQ 
241:	LGLPIALALF FEVTLFAVVA LLVSPLGIVD VAGHQIALNF SSLMFVLPMS LAAAVTIRVG 
301:	YRLGQGSTLD AQTAARTGLM VGVCMATLTA IFTVSLREQI ALLYNDNPEV VTLAAHLMLL 
361:	AAVYQISDSI QVIGSGILRG YKDTRSIFYI TFTAYWVLGL PSGYILALTD LVVEPMGPAG 
421:	FWIGFIIGLT SAAIMMMLRM RFLQRLPSAI ILQRASR