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2.A.66.2.12
Isoprenoid lipid sugar glycan flippase, Wzx (note: Wzx forms a complex with Wzy and Wzz for assembly of periplasmic O-antigen) (Marolda et al., 2006). Wzx has a 12 TMS topology (Cunneen and Reeves, 2008). WzyE (450aas; 12 TMSs; TC#9.B.128.1.1; B614D1) is called the enterobacterial common antigen (ECA) polysaccharide chain elongation polymerase (Marolda et al., 2006). The structure of Wzz has been determined by cryoEM (Collins et al. 2017).

Accession Number:Q1L811
Protein Name:Wzx
Length:411
Molecular Weight:46705.00
Species:Escherichia coli [562]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate isoprenoid lipid-sugar glycan

Cross database links:

Pfam: PF01943   

Gene Ontology

GO:0016020 C:membrane
GO:0000271 P:polysaccharide biosynthetic process

References (1)

[1] “A group of Escherichia coli and Salmonella enterica O antigens sharing a common backbone structure.”  Wang W.et.al.   17600060

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MIKEIFSDGI FSLFNKICYF GFKTLAIILI TRSLGEDVGG EFIFLVGFLE VLRVVTDFGV 
61:	DLFVIKKYND STDKIKLLNV TFAQKILSGV IVLLLFCLYS FVQGYQYNVY IPIVLALPVA 
121:	LFFNFSNSYF QSLNSNKSLT FPIAIASIAT CTIFAIQYFL NVKFNAWDYL CVELLFVSLV 
181:	TLKLFVSVKF RFIRTSAFDV LMSILSLYKY TLSIGLTAVV VIIYSRIDNF YLKTFAPGDL 
241:	AAYGQIFRMV DPLVMVSSVF STVAYARFCR INLQEKNAFT KVFQFLLCIV AYGVLSSLVY 
301:	FFIIKYLGHF VLISNVNNDF LIMMFLIVAC VKCINGGTTA IIQSQGFYKV GLYVSYVCIL 
361:	TSIPTMYFLI MKYGVKGAAM TILLVECISF LLLLCSFFMI KKMRPKKTFE M