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2.A.66.4.3
Peptidoglycan biosynthesis protein MurJ (Ruiz 2008). A 3-d structural model showed a solvent-exposed cavity within the plane of the membrane (Butler et al. 2013). MurJ has 14 TMSs, and specific charged residues localized in the central cavity are essential for function. This structural homology model suggests that MurJ functions as an essential transporter in PG biosynthesis (Butler et al. 2013). Based on an in vivo assay, MurJ is a flippase for the lipid-linked cell wall precursors, polyisoprenoid-linked disaccharide-peptapeptides (Sham et al. 2014).  There is controversy about the role of this porter and FtsW/RodA which on the basis of an in vitro assay, were thought to be flippases for the same intermediate (Young 2014). MurJ, the bacterial lipid II flippase, functions by an alternating-access mechanism (Kumar et al. 2019).

Accession Number:P0AF16
Protein Name:Probable peptidoglycan biosynthesis protein MurJ
Length:511
Molecular Weight:55267.00
Species:Escherichia coli (strain K12) [83333]
Number of TMSs:13
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Peptidoglycan precursors

Cross database links:

Genevestigator: P0AF16
EchoBASE: EB3639
EcoGene: EG13880
eggNOG: COG0728
HEGENOM: HOG000263812
Entrez Gene ID: 945487   
Pfam: PF03023   
KEGG: eco:b1069   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005319 F:lipid transporter activity
GO:0015648 F:lipid-linked peptidoglycan transporter activity
GO:0034204 P:lipid translocation
GO:0009252 P:peptidoglycan biosynthetic process

References (7)

[1] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
[5] “Involvement of an essential gene, mviN, in murein synthesis in Escherichia coli.”  Inoue A.et.al.   18708495
[6] “Bioinformatics identification of MurJ (MviN) as the peptidoglycan lipid II flippase in Escherichia coli.”  Ruiz N.et.al.   18832143
[7] “Identification of FtsW as a transporter of lipid-linked cell wall precursors across the membrane.”  Mohammadi T.et.al.   21386816

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNLLKSLAAV SSMTMFSRVL GFARDAIVAR IFGAGMATDA FFVAFKLPNL LRRIFAEGAF 
61:	SQAFVPILAE YKSKQGEDAT RVFVSYVSGL LTLALAVVTV AGMLAAPWVI MVTAPGFADT 
121:	ADKFALTSQL LKITFPYILL ISLASLVGAI LNTWNRFSIP AFAPTLLNIS MIGFALFAAP 
181:	YFNPPVLALA WAVTVGGVLQ LVYQLPHLKK IGMLVLPRIN FHDAGAMRVV KQMGPAILGV 
241:	SVSQISLIIN TIFASFLASG SVSWMYYADR LMEFPSGVLG VALGTILLPS LSKSFASGNH 
301:	DEYNRLMDWG LRLCFLLALP SAVALGILSG PLTVSLFQYG KFTAFDALMT QRALIAYSVG 
361:	LIGLIVVKVL APGFYSRQDI KTPVKIAIVT LILTQLMNLA FIGPLKHAGL SLSIGLAACL 
421:	NASLLYWQLR KQKIFTPQPG WMAFLLRLVV AVLVMSGVLL GMLHIMPEWS LGTMPWRLLR 
481:	LMAVVLAGIA AYFAALAVLG FKVKEFARRT V