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2.A.7.14.1
Purine/pyrimidine organocation uptake permease, AtPUP1.  A thaliana has 15 paralogues, AtPUP1 to AtPUP15 (Gillissen et al. 2000). PUP1 transports adenine and cytosine with high affinity by a pmf-dependent mechanism. Purine derivatives (e.g., hypoxanthine), phytohormones (e.g., zeatin and kinetin), and alkaloids (e.g., caffeine) are potent competitive inhibitors of adenine and cytosine uptake and are probably substrates (Gillissen et al. 2000).

Accession Number:Q9FZ96
Protein Name:Purine permease 1
Length:356
Molecular Weight:39280.00
Species: [3702]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Alkaloids, Caffeine, Cytosine, Adenine, Phytohormones, kinetin, zeatin, Hypoxanthine, Purines, Pyrimidines

Cross database links:

Genevestigator: Q9FZ96
HEGENOM: HBG590489
RefSeq: NP_174144.1   
Entrez Gene ID: 839717   
Pfam: PF00892    PF03151   
BioCyc: MetaCyc:MONOMER-14768   
KEGG: ath:AT1G28230   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0015211 F:purine nucleoside transmembrane transporter...
GO:0005345 F:purine transmembrane transporter activity
GO:0010184 P:cytokinin transport

References (5)

[1] “A new family of high-affinity transporters for adenine, cytosine, and purine derivatives in Arabidopsis.”  Gillissen B.et.al.   10662864
[2] “Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.”  Theologis A.et.al.   11130712
[3] “Empirical analysis of transcriptional activity in the Arabidopsis genome.”  Yamada K.et.al.   14593172
[4] “Functional annotation of a full-length Arabidopsis cDNA collection.”  Seki M.et.al.   11910074
[5] “Transport of cytokinins mediated by purine transporters of the PUP family expressed in phloem, hydathodes, and pollen of Arabidopsis.”  Buerkle L.et.al.   12662305

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKNGLIIINC IILTIGTCGG PLLTRLYFTN GGKRIWFMSF LSTAGFPIIL IPLLVSFLSR 
61:	RRSNRNPNNA ENKRKTKLFL METPLFIASI VIGLLTGLDN YLYSYGLAYL PVSTSSLIIG 
121:	TQLAFNALFA FLLVKQKFTP FSINAVVLLT VGIGILALHS DGDKPAKESK KEYVVGFLMT 
181:	VVAALLYAFI LPLVELTYKK ARQEITFPLV LEIQMVMCLA ATFFCVIGMF IVGDFKVIAR 
241:	EAREFKIGGS VFYYALIVIT GIIWQGFFLG AIGIVFCASS LASGVLISVL LPVTEVFAVV 
301:	CFREKFQAEK GVSLLLSLWG FVSYFYGEFK SGKKVVDKPQ PPETELPILP VSDYVA