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2.A.7.27.1
Csg2 (Cls2) Ca2+ homeostasis protein. Cells lacking Csg2p accumulate Ca2+ in a pool which is exchangeable with extracellular Ca2+ . The mutant cells are Ca2+ sensitive. The protein has 410 amino acyl residues, with 9-10 TMSs. It exhibits an EF-hand Ca2+ binding motif on the lumenal side of the endoplasmic reticular membrane. It is possible that it functions in Ca2+ sequestration. It regulates the activities of CSH1 and SUR1 during mannosyl phosphorylinositol ceramid synthesis. It forms heterodimers with CSH1 and SUR1 (Beeler et al. 1994; Takita et al. 1995). Cls2p likely functions in releasing Ca2+ from the endoplasmic reticulum, somehow cooperating with calcineurin (Tanida et al. 1996). It regulates the transport and protein leves of the inositol phosphorlyceramide mannosyltransferases Csg1 and Csh1 (Uemura et al. 2007).

Accession Number:P35206
Protein Name:Mannosyl phosphorylinositol ceramide synthase regulatory protein CSG2 precursor
Length:410
Molecular Weight:45515.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:9
Location1 / Topology2 / Orientation3: Endoplasmic reticulum membrane1 / Multi-pass membrane protein2
Substrate Ca2+

Cross database links:

Genevestigator: P35206
eggNOG: fuNOG14013
HEGENOM: HBG204127
DIP: DIP-8095N
RefSeq: NP_009592.1   
Entrez Gene ID: 852324   
KEGG: sce:YBR036C   

Gene Ontology

GO:0030176 C:integral to endoplasmic reticulum membrane
GO:0030234 F:enzyme regulator activity
GO:0005515 F:protein binding
GO:0006874 P:cellular calcium ion homeostasis
GO:0042493 P:response to drug

References (7)

[1] “A novel protein, CSG2p, is required for Ca2+ regulation in Saccharomyces cerevisiae.”  Beeler T.J.et.al.   8125941
[2] “The CLS2 gene encodes a protein with multiple membrane-spanning domains that is important Ca2+ tolerance in yeast.”  Takita Y.et.al.   7854312
[3] “Complete DNA sequence of yeast chromosome II.”  Feldmann H.et.al.   7813418
[4] “The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene.”  Smits P.H.M.et.al.   8091864
[5] “Accumulation of the cytochrome c oxidase subunits I and II in yeast requires a mitochondrial membrane-associated protein, encoded by the nuclear SCO1 gene.”  Schulze M.et.al.   2543907
[6] “Csg1p and newly identified Csh1p function in mannosylinositol phosphorylceramide synthesis by interacting with Csg2p.”  Uemura S.et.al.   12954640
[7] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258

External Searches:

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSTTLLWFSS VIGYVIQTKC LSNIQSKKEI SVGPNGTIAT PETNGDNGNS SSLTFYLTFM 
61:	YFASWLLWVP ASRLWEKMRP MFVSDSDSNR NSQFDNNNSG SVTNEDVDTF SHVLDDPQPR 
121:	IPAQQQKQKI ISVATFKYVA KLTVLALIMI VADLTYNMAL SLSPAFDVAL MQNTAIFEIV 
181:	TLLYGVCGIS RKNYVFRNFL IMMNAVIGIL IISYTKATCD MLAGKLSVNP NTGELSDPFL 
241:	FDRLKGALIC GLGALIMGPF AVLWNRWFCS NISKNENSAV VLVKQSTHMA LIGIIGMVIL 
301:	LPFIPKFPSR ESVESISLFY NDKSFWFSLL GSIIFGSLPS LISILELNRK APAEYLTTCN 
361:	LGAIIFMGLA EWVCEPTQTT IVRWEVIGYI MLTVSLLVLS VTLGEGKYHH