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2.A.7.3.2
YdeD (EamA) efflux pump for O-acetylserine, cysteine, asparagine and glutamine (Dassler et al., 2000; Franke et al. 2003)

Accession Number:P31125
Protein Name:Probable amino-acid metabolite efflux pump
Length:299
Molecular Weight:32324.00
Species:Escherichia coli (strain K12) [83333]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate O-acetylserine, Cysteine, Asparagine, Glutamine

Cross database links:

Genevestigator: P31125 P31125 P31125
EchoBASE: EB1594 EB1594 EB1594
EcoGene: EG11639 EG11639 EG11639
eggNOG: COG0697 COG0697 COG0697
HEGENOM: HBG673629 HBG673629 HBG673629
Entrez Gene ID: 946081   
Pfam: PF00892   
KEGG: ecj:JW5250    eco:b1533    ecj:JW5250    eco:b1533   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015562 F:efflux transmembrane transporter activity
GO:0033228 P:cysteine export

References (12)

[1] “Genetic and functional analysis of the multiple antibiotic resistance (mar) locus in Escherichia coli.”  Cohen S.P.et.al.   8383113
[2] “A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.”  Aiba H.et.al.   9097039
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Identification of a major facilitator protein from Escherichia coli involved in efflux of metabolites of the cysteine pathway.”  Dassler T.et.al.   10844694
[6] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
[7] “Genetic and functional analysis of the multiple antibiotic resistance (mar) locus in Escherichia coli.”  Cohen S.P.et.al.   8383113
[8] “A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.”  Aiba H.et.al.   9097039
[9] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[10] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[11] “Identification of a major facilitator protein from Escherichia coli involved in efflux of metabolites of the cysteine pathway.”  Dassler T.et.al.   10844694
[12] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSRKDGVLAL LVVVVWGLNF VVIKVGLHNM PPLMLAGLRF MLVAFPAIFF VARPKVPLNL 
61:	LLGYGLTISF AQFAFLFCAI NFGMPAGLAS LVLQAQAFFT IMLGAFTFGE RLHGKQLAGI 
121:	ALAIFGVLVL IEDSLNGQHV AMLGFMLTLA AAFSWACGNI FNKKIMSHST RPAVMSLVIW 
181:	SALIPIIPFF VASLILDGSA TMIHSLVTID MTTILSLMYL AFVATIVGYG IWGTLLGRYE 
241:	TWRVAPLSLL VPVVGLASAA LLLDERLTGL QFLGAVLIMT GLYINVFGLR WRKAVKVGS