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2.A.7.3.75
SepJ, a novel composite protein of 751 aas needed for cellular filament integrity, proper heterocyst development and N2 fixation. It has a C-terminal DME family domain (Flores et al., 2007). Mullineaux et al. (2008) have proposed that this protein (SepJ; FraG) may be a channel-forming protein for transfer of metabolites between cells.  However, it may instead be a polar amino acid transporter since DmeA of Synecococcus (TC# 2.A.7.3.58) complements a defect in SepJ (E. Flores, unpubished observations).

Accession Number:Q8YUK6
Protein Name:SepJ
Length:751
Molecular Weight:81389.00
Species:Anabaena sp. (strain PCC 7120) [103690]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate

Cross database links:

eggNOG: COG0697
HEGENOM: HBG580888
RefSeq: NP_486378.1   
Entrez Gene ID: 1105935   
Pfam: PF00892    PF03151   
BioCyc: NSP103690:ALR2338-MONOMER   
KEGG: ana:alr2338   

Gene Ontology

GO:0016020 C:membrane

References (1)

[1] “Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120.”  Kaneko T.et.al.   11759840

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MGRFEKRPDN DPRVRGELSR AAETALWAVV EDLESLQQNV LRSFQEEIKK LQTEKDRLTD 
61:	EVQQLIEEKE HLQEVRRITE QQVLIRQLSE ALAKHICSQL QSSLAKIANQ TESQIAALKS 
121:	AQSIGPAIEN NEQVEKMLGS LDDNLTIAFN SLQQELKNYQ SNLSQQLSRM YNQQQQGETI 
181:	VEELIDRLRG ELTRAIQETS TAKAQLSPPT VLQPPELQPP SSPVVVNLSP PTVLQFPDQQ 
241:	SPNPLQASTP LEETSTTKPS VSITPPEKST PVTIVPPPQE TRPETKSVIP KVSPDSETKL 
301:	QSSQEKAAEP SSVINRELSA GAAKSPLTPE KPPEPISTSK TKFSPSSEKP PEPISTSKTK 
361:	FSPSSEKPPE PVSVLSRDSS ASKASTPPPA PVVRRGSTPS SSRSRKSSNL SPVQVGFLLV 
421:	VLSTVMTALY NVVLKGMFYK TSQLSAMLEV AGLISPTLGN IMLILTLRLM VVVPLMILLA 
481:	PMMYPQVWQD LQNLKQSLGN NQSGSRSQPQ RFVQLMFASG CFLFLSQVLI YLAIGQVPTG 
541:	VAIALFFVYP LINGVLSWLL FRDRPGVFRA SAIGAIFCGE VLVFAGATST GIGTTPLGSI 
601:	TAILAGAAFA CYLILTRVCA AKVHPVSLSL INFVTMLGLS FIFLMIPLPE NWSLVVDPSK 
661:	LLEIVLSAFI LGALTLLSYV FSHIGINKLG GLRSAIISAS VPILTVILAG LVLQETLNIA 
721:	QIFGVLFVTF GAIAFGIGKI QNPNKPANAE G