TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.71.2.2
Folate/biopterin uptake carrier, Fbp. Transports 5-formyl tetrahydrofolate monoglutamate but not 5-formyltetrahydrofolate triglutamate. (Also transports methotrexate and aminopterin) (Klaus et al., 2005) (Nine homologues are present in Arabidopsis thaliana.) (Klaus et al., 2005). Mutational analyses suggest that the folate binding site involves the core helices 1, 4, 7 and 10 and is in the center of the membrane (Eudes et al., 2010).

Accession Number:Q9SKZ5
Protein Name:Expressed protein
Length:560
Molecular Weight:60707.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Biopterin, folate, 5-formyl tetrahydrofolate monoglutamate, methotrexate, Aminopterin

Cross database links:

Genevestigator: Q9SKZ5
eggNOG: euNOG10377
HEGENOM: HBG411578
RefSeq: NP_565734.1   
Entrez Gene ID: 817764   
Pfam: PF03092   
KEGG: ath:AT2G32040   

Gene Ontology

GO:0009941 C:chloroplast envelope
GO:0015231 F:5-formyltetrahydrofolate transporter activity

References (3)

[1] “Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.”  Lin X.et.al.   10617197
[2] “Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks.”  Reiland S.et.al.   19376835
[3] “Full-length messenger RNA sequences greatly improve genome annotation.”  Haas B.J.et.al.   12093376

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MASRSLFSIS ISPSQFTVLP SINNERRRLA IAARSHRLSR RKIRKRPPDR DMSSIVSIPE 
61:	LPRRRDSEES LLLDSRISVA EGDTSNTDVE GDRDTTSSIR TQPPPRKGRN LSSSRIKFYG 
121:	VELSPDNVAV AMVYFVQGVL GLARLAVSFY LKDDLHLDPA ETAVITGLSS LPWLVKPLYG 
181:	FISDSVPLFG YRRRSYLVLS GLLGAFSWSL MAGFVDSKYS AAFCILLGSL SVAFSDVVVD 
241:	SMVVERARGE SQSVSGSLQS LCWGSSAFGG IVSSYFSGSL VESYGVRFVF GVTALLPLIT 
301:	SAVAVLVNEQ RVVRPASGQK ENITLLSPGF LQTSKQNMIQ LWGAIKQPNV FLPTLFIFLW 
361:	QATPHSDSAM FYFTTNKLGF TPEFLGRVKL VTSIASLLGV GLYNGFLKTV PLRKIFLVTT 
421:	IFGTGLGMTQ VILVSGFNRQ LGISDEWFAI GDSLILTVLA QASFMPVLVL AARLCPEGME 
481:	ATLFATLMSI SNGGSVLGGL MGAGLTQAFG ITRDSFGNLS TLIILCNLSS LLPLPLLGLL 
541:	PRDSPDTLTK DDADVEMKSN