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2.A.72.1.1
Low affinity K+ uptake permease, KUP or TrkD.  It has 12 established TMSs with four transmembrane acidic residues important for K+ uptake (Sato et al. 2014).

Accession Number:P63183
Protein Name:Low affinity potassium transport system protein KUP aka TRKD aka B3747
Length:622
Molecular Weight:69294.00
Species:Escherichia coli [83333]
Number of TMSs:13
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate K+

Cross database links:

Genevestigator: P63183
EchoBASE: EB1503
EcoGene: EG11541
eggNOG: COG3158
HEGENOM: HBG351154
RefSeq: AP_004040.1    YP_026244.1   
Entrez Gene ID: 948255   
Pfam: PF02705   
BioCyc: EcoCyc:KUP-MONOMER   
KEGG: ecj:JW5609    eco:b3747   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015079 F:potassium ion transmembrane transporter act...
GO:0015293 F:symporter activity
GO:0006813 P:potassium ion transport

References (10)

[1] “Nucleotide sequence and 3'-end deletion studies indicate that the K(+)-uptake protein kup from Escherichia coli is composed of a hydrophobic core linked to a large and partially essential hydrophilic C-terminus.”  Schleyer M.et.al.   8226635
[2] “DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication.”  Burland V.D.et.al.   7686882
[3] “Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes.”  Blattner F.R.et.al.   8265357
[4] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[5] “Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005.”  Riley M.et.al.   16397293
[6] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[7] “Specific cesium transport via the Escherichia coli Kup (TrkD) K+ uptake system.”  Bossemeyer D.et.al.   2649491
[8] “Kup is the major K+ uptake system in Escherichia coli upon hyper-osmotic stress at a low pH.”  Trchounian A.et.al.   10214935
[9] “K+ influx by Kup in Escherichia coli is accompanied by a decrease in H+ efflux.”  Zakharyan E.et.al.   11682179
[10] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSTDNKQSLP AITLAAIGVV YGDIGTSPLY TLRECLSGQF GFGVERDAVF GFLSLIFWLL 
61:	IFVVSIKYLT FVMRADNAGE GGILTLMSLA GRNTSARTTS MLVIMGLIGG SFFYGEVVIT 
121:	PAISVMSAIE GLEIVAPQLD TWIVPLSIIV LTLLFMIQKH GTAMVGKLFA PIMLTWFLIL 
181:	AGLGLRSIIA NPEVLHALNP MWAVHFFLEY KTVSFIALGA VVLSITGVEA LYADMGHFGK 
241:	FPIRLAWFTV VLPSLTLNYF GQGALLLKNP EAIKNPFFLL APDWALIPLL IIAALATVIA 
301:	SQAVISGVFS LTRQAVRLGY LSPMRIIHTS EMESGQIYIP FVNWMLYVAV VIVIVSFEHS 
361:	SNLAAAYGIA VTGTMVLTSI LSTTVARQNW HWNKYFVALI LIAFLCVDIP LFTANLDKLL 
421:	SGGWLPLSLG TVMFIVMTTW KSERFRLLRR MHEHGNSLEA MIASLEKSPP VRVPGTAVYM 
481:	SRAINVIPFA LMHNLKHNKV LHERVILLTL RTEDAPYVHN VRRVQIEQLS PTFWRVVASY 
541:	GWRETPNVEE VFHRCGLEGL SCRMMETSFF MSHESLILGK RPWYLRLRGK LYLLLQRNAL 
601:	RAPDQFEIPP NRVIELGTQV EI