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2.A.8.1.5
The D-serine transporter, DsdX (KM=60µM) (may also transport D-threonine which inhibits D-serine uptake) (Anfora and Welch, 2006). Eliminating the dsdX gene renders the cell more sensitive to chloramphenicol (Duo et al. 2008).

Accession Number:P08555
Protein Name:DsdX permease
Length:445
Molecular Weight:47163.00
Species:Escherichia coli (strain K12) [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Serine

Cross database links:

Genevestigator: P08555
EchoBASE: EB0246
EcoGene: EG10250
eggNOG: COG2610
HEGENOM: HBG649941
DIP: DIP-9481N
RefSeq: AP_002965.1    NP_416866.1   
Entrez Gene ID: 949103   
Pfam: PF02447   
BioCyc: EcoCyc:DSDX-MONOMER    ECOL168927:B2365-MONOMER   
KEGG: ecj:JW2362    eco:b2365   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015128 F:gluconate transmembrane transporter activity
GO:0015725 P:gluconate transport

References (9)

[1] “Organization and transcriptional regulation of the Escherichia coli K-12 D-serine tolerance locus.”  Noerregaard-Madsen M.et.al.   7592420
[2] “DNA sequence of the D-serine deaminase activator gene dsdC.”  Palchaudhuri S.et.al.   3275618
[3] “Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.”  Yamamoto Y.et.al.   9205837
[4] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[5] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[6] “In vivo D-serine deaminase transcription start sites in wild-type Escherichia coli and in dsdA promoter mutants.”  Bornstein-Forst S.M.et.al.   3029015
[7] “Analysis of the gluconate (gnt) operon of Bacillus subtilis.”  Reizer A.et.al.   1659648
[8] “Adaptation of sucrose metabolism in the Escherichia coli wild-type strain EC3132.”  Jahreis K.et.al.   12218016
[9] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MHSQIWVVST LLISIVLIVL TIVKFKFHPF LALLLASFFV GTMMGMGPLD MVNAIESGIG 
61:	GTLGFLAAVI GLGTILGKMM EVSGAAERIG LTLQRCRWLS VDVIMVLVGL ICGITLFVEV 
121:	GVVLLIPLAF SIAKKTNTSL LKLAIPLCTA LMAVHCVVPP HPAALYVANK LGADIGSVIV 
181:	YGLLVGLMAS LIGGPLFLKF LGQRLPFKPV PTEFADLKVR DEKTLPSLGA TLFTILLPIA 
241:	LMLVKTIAEL NMARESGLYI LVEFIGNPIT AMFIAVFVAY YVLGIRQHMS MGTMLTHTEN 
301:	GFGSIANILL IIGAGGAFNA ILKSSSLADT LAVILSNMHM HPILLAWLVA LILHAAVGSA 
361:	TVAMMGATAI VAPMLPLYPD ISPEIIAIAI GSGAIGCTIV TDSLFWLVKQ YCGATLNETF 
421:	KYYTTATFIA SVVALAGTFL LSFII