TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.8.1.8
Low-affinity (212 μM), gluconate-inducible, gluconate transporter (gluconate permease) (GntU/the Gnt-I system) (Tong et al. 1996). The gntU gene is in an operon with gntK, encoding a gluconate kinase (Tong et al. 1996).  This system has been utilized for the production of pyruvate from gluconate (Yang et al. 2017).

Accession Number:P0AC96
Protein Name:Low-affinity gluconate transporter
Length:446
Molecular Weight:46416.00
Species:Escherichia coli (strain K12) [83333]
Number of TMSs:13
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Gluconate

Cross database links:

Genevestigator: P0AC96
EchoBASE: EB2515
EcoGene: EG12631
eggNOG: COG2610
HEGENOM: HOG000237114
Entrez Gene ID: 2847760   
Pfam: PF02447   
KEGG: eco:b4476   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0005402 F:cation:sugar symporter activity
GO:0015128 F:gluconate transmembrane transporter activity
GO:0019521 P:D-gluconate metabolic process

References (7)

[1] “Analysis of the Escherichia coli gntT and gntU genes and comparison of the products with their homologues.”  Yamada M.et.al.   8987614
[2] “Gene organization and transcriptional regulation of the gntRKU operon involved in gluconate uptake and catabolism of Escherichia coli.”  Izu H.et.al.   9135111
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005.”  Riley M.et.al.   16397293
[5] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[6] “Cloning and molecular genetic characterization of the Escherichia coli gntR, gntK, and gntU genes of GntI, the main system for gluconate metabolism.”  Tong S.et.al.   8655507
[7] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MTTLTLVLTA VGSVLLLLFL VMKARMHAFL ALMVVSMGAG LFSGMPLDKI AATMEKGMGG 
61:	TLGFLAVVVA LGAMFGKILH ETGAVDQIAV KMLKSFGHSR AHYAIGLAGL VCALPLFFEV 
121:	AIVLLISVAF SMARHTGTNL VKLVIPLFAG VAAAAAFLVP GPAPMLLASQ MNADFGWMIL 
181:	IGLCAAIPGM IIAGPLWGNF ISRYVELHIP DDISEPHLGE GKMPSFGFSL SLILLPLVLV 
241:	GLKTIAARFV PEGSTAYEWF EFIGHPFTAI LVACLVAIYG LAMRQGMPKD KVMEICGHAL 
301:	QPAGIILLVI GAGGVFKQVL VDSGVGPALG EALTGMGLPI AITCFVLAAA VRIIQGSATV 
361:	ACLTAVGLVM PVIEQLNYSG AQMAALSICI AGGSIVVSHV NDAGFWLFGK FTGATEAETL 
421:	KTWTMMETIL GTVGAIVGMI AFQLLS