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2.A.85.6.1
MdtO (YjcQ), Multidrug resistance protein (involved in resistance to puromycin, acriflavin and tetraphenyl arsonium chloride; acts with MdtN (TC# 8.A.1.1.3) and MdtP (TC# 1.B.17.3.9)) (Sulavik et al., 2001).

Accession Number:P32715
Protein Name:Multidrug resistance protein mdtO
Length:683
Molecular Weight:76151.00
Species:Escherichia coli (strain K12) [83333]
Number of TMSs:9
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate puromycin, acriflavin, Tetraphenyl arsonium chloride

Cross database links:

Genevestigator: P32715
EchoBASE: EB1896
EcoGene: EG11953
eggNOG: COG1289
HEGENOM: HBG467485
DIP: DIP-12556N
RefSeq: AP_004582.1    NP_418505.2   
Entrez Gene ID: 948582   
Pfam: PF04632   
BioCyc: EcoCyc:YJCQ-MONOMER    ECOL168927:B4081-MONOMER   
KEGG: ecj:JW4042    eco:b4081   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015559 ?:?
GO:0015546 F:sulfathiazole transporter activity
GO:0046677 P:response to antibiotic
GO:0015906 P:sulfathiazole transport

References (5)

[1] “Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes.”  Blattner F.R.et.al.   8265357
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Antibiotic susceptibility profiles of Escherichia coli strains lacking multidrug efflux pump genes.”  Sulavik M.C.et.al.   11257026
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSALNSLPLP VVRLLAFFHE ELSERRPGRV PQTVQLWVGC LLVILISMTF EIPFVALSLA 
61:	VLFYGIQSNA FYTKFVAILF VVATVLEIGS LFLIYKWSYG EPLIRLIIAG PILMGCMFLM 
121:	RTHRLGLVFF AVAIVAIYGQ TFPAMLDYPE VVVRLTLWCI VVGLYPTLLM TLIGVLWFPS 
181:	RAISQMHQAL NDRLDDAISH LTDSLAPLPE TRIEREALAL QKLNVFCLAD DANWRTQNAW 
241:	WQSCVATVTY IYSTLNRYDP TSFADSQAII EFRQKLASEI NKLQHAVAEG QCWQSDWRIS 
301:	ESEAMAAREC NLENICQTLL QLGQMDPNTP PTPAAKPPSM AADAFTNPDY MRYAVKTLLA 
361:	CLICYTFYSG VDWEGIHTCM LTCVIVANPN VGSSYQKMVL RFGGAFCGAI LALLFTLLVM 
421:	PWLDNIVELL FVLAPIFLLG AWIATSSERS SYIGTQMVVT FALATLENVF GPVYDLVEIR 
481:	DRALGIIIGT VVSAVIYTFV WPESEARTLP QKLAGTLGML SKVMRIPRQQ EVTALRTYLQ 
541:	IRIGLHAAFN ACEEMCQRVA LERQLDSEER ALLIERSQTV IRQGRDLLHA WDATWNSAQA 
601:	LDNALQPDRA GQFADALEKY AAGLATALSR SPQITLEETP ASQAILPTLL KQEQHVCQLF 
661:	ARLPDWTAPA LTPATEQAQG ATQ