TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.96.1.1
Acetate/succinate transporter, SatP or YaaH (Sá-Pessoa et al. 2013).  It is specific for acetate (a monocarboxylate) and for succinate (a dicarboxylate), with affinity constants at pH 6.0 of 1.24 ± 0.13 mM for acetate and 1.18 ± 0.10 mM for succinate. In glucose grown-cells, the ΔyaaH mutant is compromised for the uptake of both labelled acetic and succinic acids. YaaH, together with ActP, previously described as an acetate transporter, affect the use of acetic acid as sole carbon and energy source. Both genes have to be deleted simultaneously to abolish acetate transport. The uptake of acetate and succinate was restored when yaaH was expressed in trans in ΔyaaH ΔactP cells. YaaH amino acid residues Leu131 and Ala164 are important for the enhanced ability to transport lactate (Sá-Pessoa et al. 2013). The 3-d structure has been determined to 2.8 Å resolution showing that it is a hexamer with each protomer having a central pore surrounded by 6 TMSs (Sun et al. 2018) (see family description for more details). Wu et al. 2019 suggested that channel opeining involves repacking of key residues, such as Gln50 and Phe17, as well as the subsequent outward movement of all transmembrane helices. Their simulations suggested that acetate is always surrounded by several water molecules when passing through the channel, and a high energy barrier of 15 kcal/mol was observed (Wu et al. 2019).

Accession Number:P0AC98
Protein Name:Inner membrane protein YaaH aka B0010
Length:188
Molecular Weight:20071.00
Species:Escherichia Coli strain K-12 [83333]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Acetate, Succinate

Cross database links:

Genevestigator: P0AC98
EchoBASE: EB1474
EcoGene: EG11512
eggNOG: COG1584
HEGENOM: HBG736287
RefSeq: AP_000674.1    NP_414551.1   
Entrez Gene ID: 944792   
Pfam: PF01184   
BioCyc: EcoCyc:EG11512-MONOMER    ECOL168927:B0010-MONOMER   
KEGG: ecj:JW0009    eco:b0010   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane

References (5)

[1] “Five open reading frames upstream of the dnaK gene of E. coli.”  James R.et.al.   8400364
[2] “Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region.”  Yura T.et.al.   1630901
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MGNTKLANPA PLGLMGFGMT TILLNLHNVG YFALDGIILA MGIFYGGIAQ IFAGLLEYKK 
61:	GNTFGLTAFT SYGSFWLTLV AILLMPKLGL TDAPNAQFLG VYLGLWGVFT LFMFFGTLKG 
121:	ARVLQFVFFS LTVLFALLAI GNIAGNAAII HFAGWIGLIC GASAIYLAMG EVLNEQFGRT 
181:	VLPIGESH