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2.A.96.1.5
The ammonium exporter (ammonium outward transporter 2), Ato2 or Fun34 (Guaragnella and Butow, 2003; Ricicová et al., 2007)

Accession Number:Q12359
Protein Name:Ammonia transport outward protein 3
Length:275
Molecular Weight:30028.00
Species:Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [559292]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Ammonium

Cross database links:

Genevestigator: Q12359
eggNOG: fuNOG09007
HEGENOM: HBG736287
DIP: DIP-4513N
Entrez Gene ID: 851992   
Pfam: PF01184   
KEGG: sce:YDR384C   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005739 C:mitochondrion
GO:0005886 C:plasma membrane
GO:0008519 F:ammonium transmembrane transporter activity
GO:0019740 P:nitrogen utilization

References (9)

[1] “The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.”  Jacq C.et.al.   9169867
[2] “Ammonia pulses and metabolic oscillations guide yeast colony development.”  Palkova Z.et.al.   12429834
[3] “Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae.”  Ficarro S.B.et.al.   11875433
[4] “ATO3 encoding a putative outward ammonium transporter is an RTG-independent retrograde responsive gene regulated by GCN4 and the Ssy1-Ptr3-Ssy5 amino acid sensor system.”  Guaragnella N.et.al.   12966084
[5] “Combined global localization analysis and transcriptome data identify genes that are directly coregulated by Adr1 and Cat8.”  Tachibana C.et.al.   15743812
[6] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
[7] “Association of putative ammonium exporters Ato with detergent-resistant compartments of plasma membrane during yeast colony development: pH affects Ato1p localisation in patches.”  Ricicova M.et.al.   17395151
[8] “Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase.”  Reinders J.et.al.   17761666
[9] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
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FASTA formatted sequence
1:	MTSSASSPQD LEKGVNTLEN IETLPQQGSI AGVSQGFPNI QEIYSDRDFI TLGSSTYRRR 
61:	DLLNALDRGD GEEGNCAKYT PHQFANPVPL GLASFSLSCL VLSLINANVR GVTDGKWALS 
121:	LFMFFGGAIE LFAGLLCFVI GDTYAMTVFS SFGGFWICYG YGLTDTDNLV SGYTDPTMLN 
181:	NVIGFFLAGW TVFTFLMLMC TLKSTWGLFL LLTFLDLTFL LLCIGTFIDN NNLKMAGGYF 
241:	GILSSCCGWY SLYCSVVSPS NSYLAFRAHT MPNAP