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3.A.1.111.3
Nisin exporter

Accession Number:Q03203
Protein Name:NisT
Length:600
Molecular Weight:69210.00
Species:Lactococcus lactis (subsp [1360]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Nisin

Cross database links:

Pfam: PF00005   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005524 F:ATP binding
GO:0042626 F:ATPase activity, coupled to transmembrane m...
GO:0043213 P:bacteriocin transport
GO:0055085 P:transmembrane transport

References (3)

[1] “Biosynthesis of the lantibiotic nisin: genomic organization and membrane localization of the NisB protein.”  Engelke G.et.al.   1482192
[2] “Characterization of the nisin gene cluster nisABTCIPR of Lactococcus lactis. Requirement of expression of the nisA and nisI genes for development of immunity.”  Kuipers O.P.et.al.   7689965
[3] “Characterization of the nisin gene as part of a polycistronic operon in the chromosome of Lactococcus lactis ATCC 11454.”  Steen M.T.et.al.   1905517

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MDEVKEFTSK QFFYTLLTLP STLKLIFQLE KRYAIYLIVL NAITAFVPLA SLFIYQDLIN 
61:	SVLGSGRHLI NIIIIYFIVQ VITTVLGQLE SYVSGKFDMR LSYSINMRLM RTTSSLELSD 
121:	YEQADMYNII EKVTQDSTYK PFQLFNAIIV ELSSFISLLS SLFFIGTWNI GVAILLLIVP 
181:	VLSLVLFLRV GQLEFLIQWQ RASSERETWY IVYLLTHDFS FKEIKLNNIS NYFIHKFGKL 
241:	KKGFINQDLA IARKKTYFNI FLDFILNLIN ILTIFAMILS VRAGKLLIGN LVSLIQAISK 
301:	INTYSQTMIQ NIYIIYNTSL FMEQLFEFLK RESVVHKKIE DTEICNQHIG TVKVINLSYV 
361:	YPNSNAFALK NINLSFEKGE LTAIVGKNGS GKSTLVKIIS GLYQPTMGII QYDKMRSSLM 
421:	PEEFYQKNIS VLFQDFVKYE LTIRENIGLS DLSSQWEDEK IIKVLDNLGL DFLKTNNQYV 
481:	LDTQLGNWFQ EGHQLSGGQW QKIALARTFF KKASIYILDE PSAALDPVAE KEIFDYFVAL 
541:	SENNISIFIS HSLNAARKAN KIVVMKDGQV EDVGSHDVLL RRCQYYQELY YSEQYEDNDE