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3.A.1.113.3
The multidrug/microcin J25 (MccJ25; 21 aa cyclic peptide antibiotic; the precursor peptide is McjA) exporter, YojI (Delgado et al., 2005). TolC is also required for export; Vincent and Morero, 2009). This system exports many phytol derivatives (Upadhyay et al. 2014).  Also exports L-cysteine (Yamada et al., 2006).  This is one of two microcin J25 exporters, the other being McjD (TC# 3.A.1.118.1).

Accession Number:P33941
Protein Name:ABC transporter ATP-binding protein, YojI
Length:547
Molecular Weight:61553.00
Species:Escherichia coli [83333]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Microcin

Cross database links:

Genevestigator: P33941
EchoBASE: EB1997
EcoGene: EG12070
eggNOG: COG4615
HEGENOM: HBG360569
RefSeq: AP_002807.1    NP_416715.1   
Entrez Gene ID: 946705   
Pfam: PF00005   
BioCyc: EcoCyc:YOJI-MONOMER    ECOL168927:B2211-MONOMER   
KEGG: ecj:JW2199    eco:b2211   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005524 F:ATP binding
GO:0042626 F:ATPase activity, coupled to transmembrane m...
GO:0015197 F:peptide transporter activity
GO:0015833 P:peptide transport
GO:0046677 P:response to antibiotic
GO:0055085 P:transmembrane transport

References (4)

[1] “A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.”  Itoh T.et.al.   9097040
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “YojI of Escherichia coli functions as a microcin J25 efflux pump.”  Delgado M.A.et.al.   15866933

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MELLVLVWRQ YRWPFISVMA LSLASAALGI GLIAFINQRL IETADTSLLV LPEFLGLLLL 
61:	LMAVTLGSQL ALTTLGHHFV YRLRSEFIKR ILDTHVERIE QLGSASLLAG LTSDVRNITI 
121:	AFVRLPELVQ GIILTIGSAA YLWMLSGKML LVTAIWMAIT IWGGFVLVAR VYKHMATLRE 
181:	TEDKLYTDFQ TVLEGRKELT LNRERAEYVF NNLYIPDAQE YRHHIIRADT FHLSAVNWSN 
241:	IMMLGAIGLV FWMANSLGWA DTNVAATYSL TLLFLRTPLL SAVGALPTLL TAQVAFNKLN 
301:	KFALAPFKAE FPRPQAFPNW QTLELRNVTF AYQDNAFSVG PINLTIKRGE LLFLIGGNGS 
361:	GKSTLAMLLT GLYQPQSGEI LLDGKPVSGE QPEDYRKLFS AVFTDVWLFD QLLGPEGKPA 
421:	NPQLVEKWLA QLKMAHKLEL SNGRIVNLKL SKGQKKRVAL LLALAEERDI ILLDEWAADQ 
481:	DPHFRREFYQ VLLPLMQEMG KTIFAISHDD HYFIHADRLL EMRNGQLSEL TGEERDAASR 
541:	DAVARTA