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3.A.1.155.1
The phage infection protein of 901 aas, PIP (Geller et al. 1993). The PIP family (3.A.1.155) includes large proteins with 1 N-terminal hydrophobic TMS, a hydrophilic domain of variable length, and 5 C-terminal putative TMSs. The functionally characterized protein from Lactococcus lactis is of 901 aas (Geller et al., 1993). Homologues obtained with one PSI-BLAST iteration include members of the MmpL family of the RND superfamily (e.g., a Bacillus protein, gi#89208076; 1038 aas). With poorer scores, a protein annotated as an ABC-2-like sequence (gi#89200681; 392 aas with 1 TMS followed by a 150 residue hydrophilic domain followed by a C-terminal 5 putative TMSs) was retrieved. Another protein annotated as ABC-2 was smaller with 6 putative TMSs in a 2 + 3 + 1 arrangement (gi#57234453; 241 aas). The hydrophilic domain in these proteins may show sequence similarity with the large periplasmic hydrophilic domains of RND porters (2.A.6.1 - 9).

Accession Number:P49022
Protein Name:Pip
Length:901
Molecular Weight:99452.00
Species:Lactococcus lactis subsp. lactis (Streptococcus lactis) [1360]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate

Cross database links:

Pfam: PF01061   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane

References (1)

[1] “Cloning of a chromosomal gene required for phage infection of Lactococcus lactis subsp. lactis C2.”  Geller B.L.et.al.   8366036

External Searches:

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  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKSIKNTWKL FALDWKRIFK NPVATFLIVA LMIIPSLYAW FNIKALWDPY ANTSQLPIAV 
61:	YSADKPASFQ DKTINIGDEV LKNLKKNHQL GWKFVDSKAE LDKGVKSGKY YAGIYLPKDF 
121:	SKDLLSFTTG DIKKPQIVYS INEKINAIAP KITSKGASSL QSQISEEFIN TASSTLLKTF 
181:	NTIGYDIDKN MVSIQKVKSA ILSTNDNLGT IDKYTQQVVD LHGKMPEIKE KLAKANEFIT 
241:	YLPEVDSLGQ KVISLNDKMP EIDKSLSLVL TLQEKIPEIQ NAGKQISMID EDFASVESTM 
301:	TQGIQEAKDG LQIINQVQKS MPDIQKLGQD ADSLGTVTLD AAKKMQSALP SITNSVQITL 
361:	QSIQEFSKNT SSVLAVIDQA IADNQLTDEE KEQINSLITN FINNIARQRE AIQNIVDYMK 
421:	QVQESNGSHD LDSTIEQLSN LDSLLSGLST RMNHLNELVQ NGDVSEIRAY LSQINDVVTK 
481:	ISNLVDNIDV SGISKTIDTV LTSLTKTIAD AKGLLNQAQQ IDFEALLNST SQTVSNAVTI 
541:	LEKYQGEMPA IKQEIHDANL MLNGNMTTIV NAINEGASLY KNEFPILKTK LGTASDFFKN 
601:	DYAGVRKDLT DTLTMANEKM PDVESALNQA NSLIQNDWPE IKSGIQKAAT AIQKGEKEVD 
661:	LGEIVKLLKL DATKESDFWT QPVEVQENQV YPIANNGSAS TPFYTALCLW VGAVLLSSLA 
721:	TTEVHLSDKD KKRYSKREQF FARMGSFVAI GLAQALIVTL GNYFGLGVDV RDPFYSVLFA 
781:	LLISIAFMIM VYVLVALFGN VGKGIAIIIL VLSISGGGGN YPIQVSGKFF QAINPYLPFT 
841:	HAVNLLRESA GGIYWPNAWF AIIILVAVSI IFVAIGAILF PHFEDKTKKI SEIGSKSHFF 
901:	H