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3.A.1.201.21
Half sized ABCB1 drug (verapamil; rhodamine 6G) exporter of specificity similar to that of P-glycoprotein (3.A.1.201.1).  The 3-d structures of the unbound (2.6 Å) and the allosteric inhibitor-bound (2.4 Å) forms have been determined (Kodan et al. 2014).  The outward opening motion is required for ATP hydrolysis. Kodan et al. 2019 have reported a pair of structures of this homodimeric P-glycoprotein: an outward-facing conformational state with bound nucleotide, and an inward-facing apo state, at resolutions of 1.9 Å and 3.0 Å, respectively. Features that can be clearly visualized include ATP binding with octahedral coordination of Mg2+; an inner chamber that significantly changes in volume with the aid of tight connections among TMSs 1, 3, and 6; a glutamate-arginine interaction that stabilizes the outward-facing conformation; and extensive interactions between TMS1 and TMS3, a property that distinguishes multidrug transporters from floppases (Kodan et al. 2019).

Accession Number:M1VAN7
Protein Name:ATP-binding cassette, sub-family B, member 1
Length:696
Molecular Weight:75350.00
Species:Cyanidioschyzon merolae strain 10D [280699]
Number of TMSs:7
Substrate verapamil, Rhodamine 6G

Cross database links:

Structure:
3WME   3WMF   3WMG     

External Searches:

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  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNAHAAQSFE TANYSSVGEN HRERTLSSSI YETDPVSPDT AVSSEATAFQ ESFPSLKLKE 
61:	KDTPNRRWWR FWARPSAAGE DPEAGDPKKA AKASGPESAY TTGVTARRIF ALAWSSSATM 
121:	IVIGFIASIL EGATLPAFAI VFGRMFQVFT KSKSQIEGET WKYSVGFVGI GVFEFIVAGS 
181:	RTALFGIASE RLARDLRVAA FSNLVEQDVT YFDRRKAGEL GGKLNNDVQV IQYSFSKLGA 
241:	VLFNLAQCVV GIIVAFIFAP ALTGVLIALS PLVVLAGAAQ MIEMSGNTKR SSEAYASAGS 
301:	VAAEVFSNIR TTKAFEAERY ETQRYGSKLD PLYRLGRRRY ISDGLFFGLS MLVIFCVYAL 
361:	ALWWGGQLIA RGSLNLGNLL TAFFSAILGF MGVGQAAQVW PDVTRGLGAG GELFAMIDRV 
421:	PQYRRPDPGA EVVTQPLVLK QGIVFENVHF RYPTRMNVEV LRGISLTIPN GKTVAIVGGS 
481:	GAGKSTIIQL LMRFYDIEPQ GGGLLLFDGT PAWNYDFHAL RSQIGLVSQE PVLFSGTIRD 
541:	NILYGKRDAT DEEVIQALRE ANAYSFVMAL PDGLDTEVGE RGLALSGGQK QRIAIARAIL 
601:	KHPTLLCLDE STSALDAESE ALVQEALDRM MASDGVTSVV IAHRLSTVAR ADLILVMQDG 
661:	VVVEQGNHSE LMALGPSGFY YQLVEKQLAS GDMSAA