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3.A.1.203.5
Peroxisomal importer, Comatose, of substrates for β-oxidation (transports fatty acids and precursors 2,4-dichlorophenoxybutyric acid (2,4-DB) and indole butyric acid (IBA) (Dietrich et al., 2009; Visser et al. 2007). The peroxisomal fatty acyl-CoA transporter, Comatose (CTS, ABCD1, ABCD1, ABCC1, PED3, Pxa1; 1337aas) (Nyathi et al., 2010) determines germination potential and fertility and is essential for acetate metabolism (Linka and Esser 2012).  It associates with long chain fatty acyl-CoA synthetases (LACS6 (Q8LPS1) and LACS7 (Q8LKS5) and has intrinsic acyl CoA thiesterase activity (De Marcos Lousa et al. 2013). It has been proposed that it transports and hydrolyzes acyl-CoA esters, releasing a non-esterified fatty acid into the peroxisomal matrix which then needs to be re-activated by peroxisomal LACS6 or LACS7 (Visser et al. 2007). Mutagenesis of three residues in TMS 9 differentially affected the ATPase and thioesterase activities (Carrier et al. 2019).

Accession Number:Q94FB9
Protein Name:ABC transporter
Length:1337
Molecular Weight:149576.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Peroxisome membrane1 / Multi-pass membrane protein2
Substrate long-chain fatty acids, 2,4-dichlorophenoxybutyric acid, Indole butyric acid

Cross database links:

Genevestigator: Q94FB9
RefSeq: NP_568072.1   
Entrez Gene ID: 830144   
Pfam: PF06472    PF00005   
KEGG: ath:AT4G39850   

Gene Ontology

GO:0046861 C:glyoxysomal membrane
GO:0009514 C:glyoxysome
GO:0016021 C:integral to membrane
GO:0005524 F:ATP binding
GO:0042626 F:ATPase activity, coupled to transmembrane m...
GO:0005325 F:peroxisomal fatty acyl CoA transporter acti...
GO:0015916 P:fatty acyl coenzyme A transport
GO:0055085 P:transmembrane transport

References (15)

[1] “The Arabidopsis pxa1 mutant is defective in an ATP-binding cassette transporter-like protein required for peroxisomal fatty acid beta-oxidation.”  Zolman B.K.et.al.   11706205
[2] “Control of germination and lipid mobilization by COMATOSE, the Arabidopsis homologue of human ALDP.”  Footitt S.et.al.   12065405
[3] “Ped3p is a peroxisomal ATP-binding cassette transporter that might supply substrates for fatty acid beta-oxidation.”  Hayashi M.et.al.   11828016
[4] “Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.”  Mayer K.F.X.et.al.   10617198
[5] “The Arabidopsis thaliana ABC protein superfamily, a complete inventory.”  Sanchez-Fernandez R.et.al.   11346655
[6] “Multifunctionality of plant ABC transporters -- more than just detoxifiers.”  Martinoia E.et.al.   11855639
[7] “2,4-Dichlorophenoxybutyric acid-resistant mutants of Arabidopsis have defects in glyoxysomal fatty acid beta-oxidation.”  Hayashi M.et.al.   9490742
[8] “The Arabidopsis COMATOSE locus regulates germination potential.”  Russell L.et.al.   10934020
[9] “Genetic analysis of indole-3-butyric acid responses in Arabidopsis thaliana reveals four mutant classes.”  Zolman B.K.et.al.   11063705
[10] “Acetate non-utilizing mutants of Arabidopsis: evidence that organic acids influence carbohydrate perception in germinating seedlings.”  Hooks M.A.et.al.   14963767
[11] “Analysis of the role of COMATOSE and peroxisomal beta-oxidation in the determination of germination potential in Arabidopsis.”  Footitt S.et.al.   16844736
[12] “The COMATOSE ATP-binding cassette transporter is required for full fertility in Arabidopsis.”  Footitt S.et.al.   17468211
[13] “Gene expression profiling reveals defined functions of the ATP-binding cassette transporter COMATOSE late in phase II of germination.”  Carrera E.et.al.   17322332
[14] “The Arabidopsis ALDP protein homologue COMATOSE is instrumental in peroxisomal acetate metabolism.”  Hooks M.A.et.al.   17581114
[15] “Plant ABC proteins - a unified nomenclature and updated inventory.”  Verrier P.J.et.al.   18299247

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MPSLQLLQLT ERGRGLVASR RKSILLAAGI VAAGGTAVYL KSRVASRRPD SSRLCNGQSD 
61:	DDETLEKLTA TDQNAKITTK KKKGGGLKSL QVLTAILLSQ MGKMGARDLL ALVATVVFRT 
121:	ALSNRLAKVQ GFLFRAAFLR RAPLFLRLIS ENIMLCFMLS TLHSTSKYIT GALSLRFRKI 
181:	LTKIIHSHYF ENMVYYKISH VDGRITHPEQ RIASDVPRFS SELSDLILDD LTAVTDGILY 
241:	AWRLCSYASP KYIFWILAYV LGAGTAIRNF SPSFGKLMSK EQQLEGEYRQ LHSRLRTHSE 
301:	SIAFYGGETR EESHIQQKFK NLVSHMSHVL HDHWWFGMIQ DFLLKYLGAT VAVILIIEPF 
361:	FSGHLRPDDS TLGRAEMLSN IRYHTSVIIS LFQALGTLSI SSRRLNRLSG YADRIHELMA 
421:	VSRELSGDDK SSFQRNRSRN YLSEANYVEF SDVKVVTPTG NVLVEDLTLR VEQGSNLLIT 
481:	GPNGSGKSSL FRVLGGLWPL VSGHIVKPGV GSDLNKEIFY VPQRPYMAVG TLRDQLIYPL 
541:	TSGQESELLT EIGMVELLKN VDLEYLLDRY QPEKEVNWGD ELSLGEQQRL GMARLFYHKP 
601:	KFAILDECTS AVTTDMEERF AAKVRAMGTS CITISHRPAL VAFHDVVLSL DGEGGWSVHY 
661:	KRDDSALLTD AEIDSVKSSD TDRQNDAMVV QRAFAAARKE SATNSKAQSY QTQLIARSPV 
721:	VDKSVVLPRF PQPQTSQRAL PSRVAAMLNV LIPTIFDKQG AQLLAVACLV VSRTLISDRI 
781:	ASLNGTTVKY VLEQDKAAFV RLIGLSVLQS GASSIIAPSL RHLTQRLALG WRIRLTQHLL 
841:	RNYLRNNAFY KVFHMSGNSI DADQRLTRDL EKLTADLSGL LTGMVKPSVD ILWFTWRMKL 
901:	LTGQRGVAIL YTYMLLGLGF LRRVAPDFGD LAGEEQQLEG KFRFMHERLN THAESIAFFG 
961:	GGAREKAMVD KKFRALLDHS LMLLRKKWLY GILDDFVTKQ LPNNVTWGLS LLYALEHKGD 
1021:	RALVSTQGEL AHALRYLASV VSQSFMAFGD ILELHKKFLE LSGGINRIFE LDEFLDASQS 
1081:	GVTSENQTSR LDSQDLLSFS EVDIITPAQK LMASKLSCEI VSGKSLLVTG PNGSGKTSVF 
1141:	RVLRDIWPTV CGRLTKPSLD IKELGSGNGM FFVPQRPYTC LGTLRDQIIY PLSKEEAEKR 
1201:	AAKLYTSGES STEAGSILDS HLKTILENVR LVYLLERDVG GWDATTNWED ILSLGEQQRL 
1261:	GMARLFFHRP KFGVLDECTN ATSVDVEEQL YRVARDMGVT FITSSQRPAL IPFHSLELRL 
1321:	IDGEGNWELR SIEQTTE