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3.A.1.205.3
Weak acid exporter, Pdr12p (exports preservative anions including propionate, sorbate and benzoate) (Mollapour et al., 2008)

Accession Number:Q02785
Protein Name:PDRC aka Pdr12p aka PDR12 aka YPL058C aka LPE14C
Length:1511
Molecular Weight:171065.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:15
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Propionate, Sorbate, Benzoate

Cross database links:

Genevestigator: Q02785
eggNOG: fuNOG05684
HEGENOM: HBG323792
RefSeq: NP_015267.1   
Entrez Gene ID: 856049   
Pfam: PF01061    PF00005    PF06422   
KEGG: sce:YPL058C   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005524 F:ATP binding
GO:0005342 F:organic acid transmembrane transporter acti...
GO:0005515 F:protein binding
GO:0008559 F:xenobiotic-transporting ATPase activity
GO:0015849 P:organic acid transport
GO:0019541 P:propionate metabolic process

References (21)

[1] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.”  Bussey H.et.al.   9169875
[2] “The pdr12 ABC transporter is required for the development of weak organic acid resistance in yeast.”  Piper P.W.et.al.   9687494
[3] “The Saccharomyces cerevisiae weak-acid-inducible ABC transporter Pdr12 transports fluorescein and preservative anions from the cytosol by an energy-dependent mechanism.”  Holyoak C.D.et.al.   10419965
[4] “Loss of Cmk1 Ca(2+)-calmodulin-dependent protein kinase in yeast results in constitutive weak organic acid resistance, associated with a post-transcriptional activation of the Pdr12 ATP-binding cassette transporter.”  Holyoak C.D.et.al.   10931353
[5] “Weak organic acid stress inhibits aromatic amino acid uptake by yeast, causing a strong influence of amino acid auxotrophies on the phenotypes of membrane transporter mutants.”  Bauer B.E.et.al.   12869194
[6] “War1p, a novel transcription factor controlling weak acid stress response in yeast.”  Kren A.et.al.   12588995
[7] “Global analysis of protein localization in budding yeast.”  Huh W.-K.et.al.   14562095
[8] “Global analysis of protein expression in yeast.”  Ghaemmaghami S.et.al.   14562106
[9] “Global phenotypic analysis and transcriptional profiling defines the weak acid stress response regulon in Saccharomyces cerevisiae.”  Schueller C.et.al.   14617816
[10] “A proteomics approach to understanding protein ubiquitination.”  Peng J.et.al.   12872131
[11] “Moderately lipophilic carboxylate compounds are the selective inducers of the Saccharomyces cerevisiae Pdr12p ATP-binding cassette transporter.”  Hatzixanthis K.et.al.   12734796
[12] “Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.”  Gruhler A.et.al.   15665377
[13] “A new physiological role for Pdr12p in Saccharomyces cerevisiae: export of aromatic and branched-chain organic acids produced in amino acid catabolism.”  Hazelwood L.A.et.al.   16911515
[14] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
[15] “A genetic screen identifies mutations in the yeast WAR1 gene, linking transcription factor phosphorylation to weak-acid stress adaptation.”  Gregori C.et.al.   17509074
[16] “High Pdr12 levels in spoilage yeast (Saccharomyces cerevisiae) correlate directly with sorbic acid levels in the culture medium but are not sufficient to provide cells with acquired resistance to the food preservative.”  Papadimitriou M.N.B.et.al.   17141908
[17] “Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.”  Li X.et.al.   17330950
[18] “Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases.”  Smolka M.B.et.al.   17563356
[19] “Pdr12p-dependent and -independent fluorescein extrusion from baker's yeast cells.”  Lushchak V.et.al.   18800175
[20] “Weak organic acids trigger conformational changes of the yeast transcription factor War1 in vivo to elicit stress adaptation.”  Gregori C.et.al.   18621731
[21] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSSTDEHIEK DISSRSNHDD DYANSVQSYA ASEGQVDNED LAATSQLSRH LSNILSNEEG 
61:	IERLESMARV ISHKTKKEMD SFEINDLDFD LRSLLHYLRS RQLEQGIEPG DSGIAFKNLT 
121:	AVGVDASAAY GPSVEEMFRN IASIPAHLIS KFTKKSDVPL RNIIQNCTGV VESGEMLFVV 
181:	GRPGAGCSTF LKCLSGETSE LVDVQGEFSY DGLDQSEMMS KYKGYVIYCP ELDFHFPKIT 
241:	VKETIDFALK CKTPRVRIDK MTRKQYVDNI RDMWCTVFGL RHTYATKVGN DFVRGVSGGE 
301:	RKRVSLVEAQ AMNASIYSWD NATRGLDAST ALEFAQAIRT ATNMVNNSAI VAIYQAGENI 
361:	YELFDKTTVL YNGRQIYFGP ADKAVGYFQR MGWVKPNRMT SAEFLTSVTV DFENRTLDIK 
421:	PGYEDKVPKS SSEFEEYWLN SEDYQELLRT YDDYQSRHPV NETRDRLDVA KKQRLQQGQR 
481:	ENSQYVVNYW TQVYYCMIRG FQRVKGDSTY TKVYLSSFLI KALIIGSMFH KIDDKSQSTT 
541:	AGAYSRGGML FYVLLFASVT SLAEIGNSFS SRPVIVKHKS YSMYHLSAES LQEIITEFPT 
601:	KFVAIVILCL ITYWIPFMKY EAGAFFQYIL YLLTVQQCTS FIFKFVATMS KSGVDAHAVG 
661:	GLWVLMLCVY AGFVLPIGEM HHWIRWLHFI NPLTYAFESL VSTEFHHREM LCSALVPSGP 
721:	GYEGISIANQ VCDAAGAVKG NLYVSGDSYI LHQYHFAYKH AWRNWGVNIV WTFGYIVFNV 
781:	ILSEYLKPVE GGGDLLLYKR GHMPELGTEN ADARTASREE MMEALNGPNV DLEKVIAEKD 
841:	VFTWNHLDYT IPYDGATRKL LSDVFGYVKP GKMTALMGES GAGKTTLLNV LAQRINMGVI 
901:	TGDMLVNAKP LPASFNRSCG YVAQADNHMA ELSVRESLRF AAELRQQSSV PLEEKYEYVE 
961:	KIITLLGMQN YAEALVGKTG RGLNVEQRKK LSIGVELVAK PSLLLFLDEP TSGLDSQSAW 
1021:	SIVQFMRALA DSGQSILCTI HQPSATLFEQ FDRLLLLKKG GKMVYFGDIG PNSETLLKYF 
1081:	ERQSGMKCGV SENPAEYILN CIGAGATASV NSDWHDLWLA SPECAAARAE VEELHRTLPG 
1141:	RAVNDDPELA TRFAASYMTQ IKCVLRRTAL QFWRSPVYIR AKFFECVACA LFVGLSYVGV 
1201:	NHSVGGAIEA FSSIFMLLLI ALAMINQLHV FAYDSRELYE VREAASNTFH WSVLLLCHAA 
1261:	VENFWSTLCQ FMCFICYYWP AQFSGRASHA GFFFFFYVLI FPLYFVTYGL WILYMSPDVP 
1321:	SASMINSNLF AAMLLFCGIL QPREKMPAFW RRLMYNVSPF TYVVQALVTP LVHNKKVVCN 
1381:	PHEYNIMDPP SGKTCGEFLS TYMDNNTGYL VNPTATENCQ YCPYTVQDQV VAKYNVKWDH 
1441:	RWRNFGFMWA YICFNIAAML ICYYVVRVKV WSLKSVLNFK KWFNGPRKER HEKDTNIFQT 
1501:	VPGDENKITK K