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3.A.1.205.9
The plasma membrane Cd2+/Pb2+ efflux pump (heavy metal resistance pump), PDR8 (ABCG36; PEN30, present in root hair and epidermal cells; it may export a broad range of substrates (Kim et al., 2007).  Also reported to transport flavonoid glycosides (phytoalexins) as well as quercitin, kaempeferol, 4-methoxy-indol-3-ylmethylglucosinolate and salicylate (Badri et al. 2012; Stein et al. 2006).  Key factor that controls the extent of cell death in the defense response (Kobae et al. 2006). Necessary for both callose deposition and glucosinolate activation in response to pathogens. Required for limiting invasion by nonadapted powdery mildews (Consonni et al. 2006).

Accession Number:Q9XIE2
Protein Name:Pleiotropic drug resistance protein 8 aka PDR8
Length:1469
Molecular Weight:165082.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:15
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate 4-methoxy-indol-3-ylmethylglucosinolate, Cd2+, Pb2+, Phytoalexins, Quercitin, Kaempeferol, Salicylate

Cross database links:

Genevestigator: Q9XIE2
HEGENOM: HBG750535
RefSeq: NP_176196.1   
Entrez Gene ID: 842281   
Pfam: PF01061    PF00005    PF08370   
KEGG: ath:AT1G59870   

Gene Ontology

GO:0009941 C:chloroplast envelope
GO:0016021 C:integral to membrane
GO:0005739 C:mitochondrion
GO:0005886 C:plasma membrane
GO:0005524 F:ATP binding
GO:0016887 F:ATPase activity
GO:0015086 F:cadmium ion transmembrane transporter activity
GO:0015691 P:cadmium ion transport
GO:0052544 P:callose deposition in cell wall during defe...
GO:0042742 P:defense response to bacterium
GO:0009817 P:defense response to fungus, incompatible in...
GO:0042344 P:indole glucosinolate catabolic process
GO:0031348 P:negative regulation of defense response
GO:0009627 P:systemic acquired resistance

References (17)

[1] “Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.”  Theologis A.et.al.   11130712
[2] “Empirical analysis of transcriptional activity in the Arabidopsis genome.”  Yamada K.et.al.   14593172
[3] “The plant PDR family of ABC transporters.”  van den Brule S.et.al.   12430018
[4] “Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database.”  Nuehse T.S.et.al.   15308754
[5] “Organization and function of the plant pleiotropic drug resistance ABC transporter family.”  Crouzet J.et.al.   16506311
[6] “Conserved requirement for a plant host cell protein in powdery mildew pathogenesis.”  Consonni C.et.al.   16732289
[7] “Arabidopsis PEN3/PDR8, an ATP binding cassette transporter, contributes to nonhost resistance to inappropriate pathogens that enter by direct penetration.”  Stein M.et.al.   16473969
[8] “Loss of AtPDR8, a plasma membrane ABC transporter of Arabidopsis thaliana, causes hypersensitive cell death upon pathogen infection.”  Kobae Y.et.al.   16415066
[9] “Mapping the Arabidopsis organelle proteome.”  Dunkley T.P.J.et.al.   16618929
[10] “Temporal analysis of sucrose-induced phosphorylation changes in plasma membrane proteins of Arabidopsis.”  Niittylae T.et.al.   17586839
[11] “Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis.”  Benschop J.J.et.al.   17317660
[12] “The ABC transporter AtPDR8 is a cadmium extrusion pump conferring heavy metal resistance.”  Kim D.-Y.et.al.   17355438
[13] “Quantitative phosphoproteomic analysis of plasma membrane proteins reveals regulatory mechanisms of plant innate immune responses.”  Nuehse T.S.et.al.   17651370
[14] “Plant ABC proteins - a unified nomenclature and updated inventory.”  Verrier P.J.et.al.   18299247
[15] “Extracellular transport and integration of plant secretory proteins into pathogen-induced cell wall compartments.”  Meyer D.et.al.   19000165
[16] “Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks.”  Reiland S.et.al.   19376835
[17] “Glucosinolate metabolites required for an Arabidopsis innate immune response.”  Clay N.K.et.al.   19095898

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FASTA formatted sequence
1:	MDYNPNLPPL GGGGVSMRRS ISRSVSRASR NIEDIFSSGS RRTQSVNDDE EALKWAAIEK 
61:	LPTYSRLRTT LMNAVVEDDV YGNQLMSKEV DVTKLDGEDR QKFIDMVFKV AEQDNERILT 
121:	KLRNRIDRVG IKLPTVEVRY EHLTIKADCY TGNRSLPTLL NVVRNMGESA LGMIGIQFAK 
181:	KAQLTILKDI SGVIKPGRMT LLLGPPSSGK TTLLLALAGK LDKSLQVSGD ITYNGYQLDE 
241:	FVPRKTSAYI SQNDLHVGIM TVKETLDFSA RCQGVGTRYD LLNELARREK DAGIFPEADV 
301:	DLFMKASAAQ GVKNSLVTDY TLKILGLDIC KDTIVGDDMM RGISGGQKKR VTTGEMIVGP 
361:	TKTLFMDEIS TGLDSSTTFQ IVKCLQQIVH LNEATVLMSL LQPAPETFDL FDDIILVSEG 
421:	QIVYQGPRDN ILEFFESFGF KCPERKGTAD FLQEVTSKKD QEQYWVNPNR PYHYIPVSEF 
481:	ASRYKSFHVG TKMSNELAVP FDKSRGHKAA LVFDKYSVSK RELLKSCWDK EWLLMQRNAF 
541:	FYVFKTVQIV IIAAITSTLF LRTEMNTRNE GDANLYIGAL LFGMIINMFN GFAEMAMMVS 
601:	RLPVFYKQRD LLFYPSWTFS LPTFLLGIPS SILESTAWMV VTYYSIGFAP DASRFFKQFL 
661:	LVFLIQQMAA SLFRLIASVC RTMMIANTGG ALTLLLVFLL GGFLLPKGKI PDWWGWAYWV 
721:	SPLTYAFNGL VVNEMFAPRW MNKMASSNST IKLGTMVLNT WDVYHQKNWY WISVGALLCF 
781:	TALFNILFTL ALTYLNPLGK KAGLLPEEEN EDADQGKDPM RRSLSTADGN RRGEVAMGRM 
841:	SRDSAAEASG GAGNKKGMVL PFTPLAMSFD DVKYFVDMPG EMRDQGVTET RLQLLKGVTG 
901:	AFRPGVLTAL MGVSGAGKTT LMDVLAGRKT GGYIEGDVRI SGFPKVQETF ARISGYCEQT 
961:	DIHSPQVTVR ESLIFSAFLR LPKEVGKDEK MMFVDQVMEL VELDSLRDSI VGLPGVTGLS 
1021:	TEQRKRLTIA VELVANPSII FMDEPTSGLD ARAAAIVMRA VRNTVDTGRT VVCTIHQPSI 
1081:	DIFEAFDELM LMKRGGQVIY AGPLGQNSHK VVEYFESFPG VSKIPEKYNP ATWMLEASSL 
1141:	AAELKLSVDF AELYNQSALH QRNKALVKEL SVPPAGASDL YFATQFSQNT WGQFKSCLWK 
1201:	QWWTYWRSPD YNLVRFIFTL ATSLLIGTVF WQIGGNRSNA GDLTMVIGAL YAAIIFVGIN 
1261:	NCSTVQPMVA VERTVFYRER AAGMYSAMPY AISQVTCELP YVLIQTVYYS LIVYAMVGFE 
1321:	WKAEKFFWFV FVSYFSFLYW TYYGMMTVSL TPNQQVASIF ASAFYGIFNL FSGFFIPRPK 
1381:	IPKWWIWYYW ICPVAWTVYG LIVSQYGDVE TRIQVLGGAP DLTVKQYIED HYGFQSDFMG 
1441:	PVAAVLIAFT VFFAFIFAFC IRTLNFQTR