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3.A.1.206.1
a-Factor sex pheromone (a hydrophobic isoprenylated (farnesylated) carboxymethylated peptide) exporter, Ste6 (Michaelis and Barrowman 2012).

Accession Number:P12866
Protein Name:Ste6 aka YKL209C
Length:1290
Molecular Weight:144766.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:13
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate a-factor sex hormone

Cross database links:

Genevestigator: P12866
eggNOG: fuNOG04952
HEGENOM: HBG396815
DIP: DIP-2594N
RefSeq: NP_012713.1   
Entrez Gene ID: 853671   
Pfam: PF00664    PF00005   
KEGG: sce:YKL209C   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0043332 C:mating projection tip
GO:0005524 F:ATP binding
GO:0042626 F:ATPase activity, coupled to transmembrane m...
GO:0005515 F:protein binding
GO:0000770 P:peptide pheromone export
GO:0019236 P:response to pheromone
GO:0055085 P:transmembrane transport

References (9)

[1] “The yeast STE6 gene encodes a homologue of the mammalian multidrug resistance P-glycoprotein.”  McGrath J.P.et.al.   2569166
[2] “Saccharomyces cerevisiae STE6 gene product: a novel pathway for protein export in eukaryotic cells.”  Kuchler K.et.al.   2686977
[3] “Complete DNA sequence of yeast chromosome XI.”  Dujon B.et.al.   8196765
[4] “Sequences upstream of the STE6 gene required for its expression and regulation by the mating type locus in Saccharomyces cerevisiae.”  Wilson K.L.et.al.   3517872
[5] “Mutational analysis of the yeast a-factor transporter STE6, a member of the ATP binding cassette (ABC) protein superfamily.”  Berkover C.et.al.   1935899
[6] “The ABC-transporter Ste6 accumulates in the plasma membrane in a ubiquitinated form in endocytosis mutants.”  Koelling R.et.al.   8045256
[7] “Comparative topology studies in Saccharomyces cerevisiae and in Escherichia coli. The N-terminal half of the yeast ABC protein Ste6.”  Geller D.et.al.   8662764
[8] “A proteomics approach to understanding protein ubiquitination.”  Peng J.et.al.   12872131
[9] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNFLSFKTTK HYHIFRYVNI RNDYRLLMIM IIGTVATGLV PAITSILTGR VFDLLSVFVA 
61:	NGSHQGLYSQ LVQRSMAVMA LGAASVPVMW LSLTSWMHIG ERQGFRIRSQ ILEAYLEEKP 
121:	MEWYDNNEKL LGDFTQINRC VEELRSSSAE ASAITFQNLV AICALLGTSF YYSWSLTLII 
181:	LCSSPIITFF AVVFSRMIHV YSEKENSETS KAAQLLTWSM NAAQLVRLYC TQRLERKKFK 
241:	EIILNCNTFF IKSCFFVAAN AGILRFLTLT MFVQGFWFGS AMIKKGKLNI NDVITCFHSC 
301:	IMLGSTLNNT LHQIVVLQKG GVAMEKIMTL LKDGSKRNPL NKTVAHQFPL DYATSDLTFA 
361:	NVSFSYPSRP SEAVLKNVSL NFSAGQFTFI VGKSGSGKST LSNLLLRFYD GYNGSISING 
421:	HNIQTIDQKL LIENITVVEQ RCTLFNDTLR KNILLGSTDS VRNADCSTNE NRHLIKDACQ 
481:	MALLDRFILD LPDGLETLIG TGGVTLSGGQ QQRVAIARAF IRDTPILFLD EAVSALDIVH 
541:	RNLLMKAIRH WRKGKTTIIL THELSQIESD DYLYLMKEGE VVESGTQSEL LADPTTTFST 
601:	WYHLQNDYSD AKTIVDTETE EKSIHTVESF NSQLETPKLG SCLSNLGYDE TDQLSFYEAI 
661:	YQKRSNVRTR RVKVEEENIG YALKQQKNTE SSTGPQLLSI IQIIKRMIKS IRYKKILILG 
721:	LLCSLIAGAT NPVFSYTFSF LLEGIVPSTD GKTGSSHYLA KWSLLVLGVA AADGIFNFAK 
781:	GFLLDCCSEY WVMDLRNEVM EKLTRKNMDW FSGENNKASE ISALVLNDLR DLRSLVSEFL 
841:	SAMTSFVTVS TIGLIWALVS GWKLSLVCIS MFPLIIIFSA IYGGILQKCE TDYKTSVAQL 
901:	ENCLYQIVTN IKTIKCLQAE FHFQLTYHDL KIKMQQIASK RAIATGFGIS MTNMIVMCIQ 
961:	AIIYYYGLKL VMIHEYTSKE MFTTFTLLLF TIMSCTSLVS QIPDISRGQR AASWIYRILD 
1021:	EKHNTLEVEN NNARTVGIAG HTYHGKEKKP IVSIQNLTFA YPSAPTAFVY KNMNFDMFCG 
1081:	QTLGIIGESG TGKSTLVLLL TKLYNCEVGK IKIDGTDVND WNLTSLRKEI SVVEQKPLLF 
1141:	NGTIRDNLTY GLQDEILEIE MYDALKYVGI HDFVISSPQG LDTRIDTTLL SGGQAQRLCI 
1201:	ARALLRKSKI LILDECTSAL DSVSSSIINE IVKKGPPALL TMVITHSEQM MRSCNSIAVL 
1261:	KDGKVVERGN FDTLYNNRGE LFQIVSNQSS