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3.A.1.208.18
Vacuolar glutathione conjugate, glutathione exporter; mediates cadmium detoxification and ade2 pigmentation in vivo (Sharma et al., 2002). (Most similar to Ycf1 of S. cerevisiae (TC# 3.A.1.208.11; 41% identity))

Accession Number:P14772
Protein Name:Bile pigment transporter 1 aka BPT1
Length:1559
Molecular Weight:176874.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:17
Location1 / Topology2 / Orientation3: Vacuole membrane1 / Multi-pass membrane protein2
Substrate Glutathione conjugates, Glutathione, Cd2+, Pigments

Cross database links:

Genevestigator: P14772
eggNOG: fuNOG08179
HEGENOM: HBG758042
DIP: DIP-2866N
RefSeq: NP_013086.1   
Entrez Gene ID: 850645   
Pfam: PF00664    PF00005   
KEGG: sce:YLL015W   

Gene Ontology

GO:0000329 C:fungal-type vacuole membrane
GO:0016021 C:integral to membrane
GO:0005524 F:ATP binding
GO:0042626 F:ATPase activity, coupled to transmembrane m...
GO:0015127 F:bilirubin transmembrane transporter activity
GO:0015086 F:cadmium ion transmembrane transporter activity
GO:0015723 P:bilirubin transport
GO:0015691 P:cadmium ion transport
GO:0055085 P:transmembrane transport

References (12)

[1] “Sequence analysis of the CEN12 region of Saccharomyces cerevisiae on a 43.7 kb fragment of chromosome XII including an open reading frame homologous to the human cystic fibrosis transmembrane conductance regulator protein CFTR.”  Miosga T.et.al.   8810043
[2] “The sequence of 32kb on the left arm of yeast chromosome XII reveals six known genes, a new member of the seripauperins family and a new ABC transporter homologous to the human multidrug resistance protein.”  Purnelle B.et.al.   9046100
[3] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.”  Johnston M.et.al.   9169871
[4] “The C-terminal part of a gene partially homologous to CDC 25 gene suppresses the cdc25-5 mutation in Saccharomyces cerevisiae.”  Boy-Marcotte E.et.al.   2545538
[5] “The products of YCF1 and YLL015w (BPT1) cooperate for the ATP-dependent vacuolar transport of unconjugated bilirubin in Saccharomyces cerevisiae.”  Petrovic S.et.al.   10790694
[6] “The ATP-binding cassette (ABC) transporter Bpt1p mediates vacuolar sequestration of glutathione conjugates in yeast.”  Klein M.et.al.   12044871
[7] “Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.”  Gruhler A.et.al.   15665377
[8] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
[9] “Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.”  Li X.et.al.   17330950
[10] “Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.”  Chi A.et.al.   17287358
[11] “Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases.”  Smolka M.B.et.al.   17563356
[12] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSSLEVVDGC PYGYRPYPDS GTNALNPCFI SVISAWQAVF FLLIGSYQLW KLYKNNKVPP 
61:	RFKNFPTLPS KINSRHLTHL TNVCFQSTLI ICELALVSQS SDRVYPFILK KALYLNLLFN 
121:	LGISLPTQYL AYFKSTFSMG NQLFYYMFQI LLQLFLILQR YYHGSSNERL TVISGQTAMI 
181:	LEVLLLFNSV AIFIYDLCIF EPINELSEYY KKNGWYPPVH VLSYITFIWM NKLIVETYRN 
241:	KKIKDPNQLP LPPVDLNIKS ISKEFKANWE LEKWLNRNSL WRAIWKSFGR TISVAMLYET 
301:	TSDLLSVVQP QFLRIFIDGL NPETSSKYPP LNGVFIALTL FVISVVSVFL TNQFYIGIFE 
361:	AGLGIRGSLA SLVYQKSLRL TLAERNEKST GDILNLMSVD VLRIQRFFEN AQTIIGAPIQ 
421:	IIVVLTSLYW LLGKAVIGGL VTMAIMMPIN AFLSRKVKKL SKTQMKYKDM RIKTITELLN 
481:	AIKSIKLYAW EEPMMARLNH VRNDMELKNF RKIGIVSNLI YFAWNCVPLM VTCSTFGLFS 
541:	LFSDSPLSPA IVFPSLSLFN ILNSAIYSVP SMINTIIETS VSMERLKSFL LSDEIDDSFI 
601:	ERIDPSADER ALPAIEMNNI TFLWKSKEVL TSSQSGDNLR TDEESIIGSS QIALKNIDHF 
661:	EAKRGDLVCV VGRVGAGKST FLKAILGQLP CMSGSRDSIP PKLIIRSSSV AYCSQESWIM 
721:	NASVRENILF GHKFDQDYYD LTIKACQLLP DLKILPDGDE TLVGEKGISL SGGQKARLSL 
781:	ARAVYSRADI YLLDDILSAV DAEVSKNIIE YVLIGKTALL KNKTIILTTN TVSILKHSQM 
841:	IYALENGEIV EQGNYEDVMN RKNNTSKLKK LLEEFDSPID NGNESDVQTE HRSESEVDEP 
901:	LQLKVTESET EDEVVTESEL ELIKANSRRA SLATLRPRPF VGAQLDSVKK TAQKAEKTEV 
961:	GRVKTKIYLA YIKACGVLGV VLFFLFMILT RVFDLAENFW LKYWSESNEK NGSNERVWMF 
1021:	VGVYSLIGVA SAAFNNLRSI MMLLYCSIRG SKKLHESMAK SVIRSPMTFF ETTPVGRIIN 
1081:	RFSSDMDAVD SNLQYIFSFF FKSILTYLVT VILVGYNMPW FLVFNMFLVV IYIYYQTFYI 
1141:	VLSRELKRLI SISYSPIMSL MSESLNGYSI IDAYDHFERF IYLNYEKIQY NVDFVFNFRS 
1201:	TNRWLSVRLQ TIGATIVLAT AILALATMNT KRQLSSGMVG LLMSYSLEVT GSLTWIVRTT 
1261:	VTIETNIVSV ERIVEYCELP PEAQSINPEK RPDENWPSKG GIEFKNYSTK YRENLDPVLN 
1321:	NINVKIEPCE KVGIVGRTGA GKSTLSLALF RILEPTEGKI IIDGIDISDI GLFDLRSHLA 
1381:	IIPQDAQAFE GTVKTNLDPF NRYSEDELKR AVEQAHLKPH LEKMLHSKPR GDDSNEEDGN 
1441:	VNDILDVKIN ENGSNLSVGQ RQLLCLARAL LNRSKILVLD EATASVDMET DKIIQDTIRR 
1501:	EFKDRTILTI AHRIDTVLDS DKIIVLDQGS VREFDSPSKL LSDKTSIFYS LCEKGGYLK