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3.A.10.1.8
Vacuolar H+-PPase. 3-d structure known at 2.3 Å resolution (Charles et al., 2011; Lin et al. 2012).  Each  subunit consists of an integral membrane domain formed by 16 transmembrane helices.  imidodiphosphate is bound in the cytosolic surface of each subunit and trapped by numerous charged residues and five Mg2+ ions. A proton translocation pathway is formed by six core transmembrane helices. Proton pumping is initialized by PPi hydrolysis, and H+ is then transported into the vacuolar lumen through a pathway consisting of Arg242, Asp294, Lys742 and Glu301 (Lin et al. 2012).  Substrate binding induces changes in domain interactions (Hsu et al. 2015).

Accession Number:P21616
Protein Name:Pyrophosphate-energized vacuolar membrane proton pump
Length:765
Molecular Weight:79980.00
Species:Vigna radiata var. radiata (Mung bean) [3916]
Number of TMSs:16
Location1 / Topology2 / Orientation3: Vacuole membrane1 / Multi-pass membrane protein2
Substrate H+

Cross database links:

Pfam: PF03030   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005774 C:vacuolar membrane
GO:0009678 F:hydrogen-translocating pyrophosphatase activity
GO:0004427 F:inorganic diphosphatase activity
GO:0015992 P:proton transport

References (2)

[1] “Purification and properties of vacuolar membrane proton-translocating inorganic pyrophosphatase from mung bean.”  Maeshima M.et.al.   2555340
[2] “Localization of a carboxylic residue possibly involved in the inhibition of vacuolar H+-pyrophosphatase by N,N'-dicyclohexylcarbodi-imide.”  Yang S.J.et.al.   10477275
Structure:
4A01     

External Searches:

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  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MGAAILPDLG TEILIPVCAV IGIAFALFQW LLVSKVKLSA VRDASPNAAA KNGYNDYLIE 
61:	EEEGINDHNV VVKCAEIQNA ISEGATSFLF TEYKYVGIFM VAFAILIFLF LGSVEGFSTS 
121:	PQACSYDKTK TCKPALATAI FSTVSFLLGG VTSLVSGFLG MKIATYANAR TTLEARKGVG 
181:	KAFITAFRSG AVMGFLLAAN GLLVLYIAIN LFKIYYGDDW GGLFEAITGY GLGGSSMALF 
241:	GRVGGGIYTK AADVGADLVG KVERNIPEDD PRNPAVIADN VGDNVGDIAG MGSDLFGSYA 
301:	ESSCAALVVA SISSFGLNHE LTAMLYPLIV SSVGILVCLL TTLFATDFFE IKAVKEIEPA 
361:	LKKQLVISTV LMTIGVAVVS FVALPTSFTI FNFGVQKDVK SWQLFLCVAV GLWAGLIIGF 
421:	VTEYYTSNAY SPVQDVADSC RTGAATNVIF GLALGYKSVI IPIFAIAISI FVSFTFAAMY 
481:	GIAVAALGML STIATGLAID AYGPISDNAG GIAEMAGMSH RIRERTDALD AAGNTTAAIG 
541:	KGFAIGSAAL VSLALFGAFV SRASITTVDV LTPKVFIGLI VGAMLPYWFS AMTMKSVGSA 
601:	ALKMVEEVRR QFNTIPGLME GTAKPDYATC VKISTDASIK EMIPPGALVM LTPLVVGILF 
661:	GVETLSGVLA GSLVSGVQIA ISASNTGAWD NAKKYIEAGA SEHARSLGPK GSDCHKAAVI 
721:	GDTIGDPLKD TSGPSLNILI KLMAVESLVF APFFATHGGL LFKIF