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3.A.10.2.1
H+-translocating pyrophosphatase (PPiase)/synthase.  It has two distinct roles depending on its location, acting as a PPi hydrolyzing intracellular proton pump in acidocalcisomes but as a PPi synthetase in the chromatophore membranes (Seufferheld et al. 2004).

Accession Number:O68460
Protein Name:RRPP
Length:702
Molecular Weight:71547.00
Species:Rhodospirillum rubrum [269796]
Number of TMSs:16
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate H+

Cross database links:

eggNOG: COG3808
HEGENOM: HBG593668
RefSeq: YP_426905.1   
Entrez Gene ID: 3835242   
Pfam: PF03030   
BioCyc: RRUB269796:RRU_A1818-MONOMER   
KEGG: rru:Rru_A1818   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0009678 F:hydrogen-translocating pyrophosphatase acti...
GO:0004427 F:inorganic diphosphatase activity
GO:0000287 F:magnesium ion binding
GO:0015992 P:proton transport

References (3)

[1] “A pyrophosphate synthase gene: molecular cloning and sequencing of the cDNA encoding the inorganic pyrophosphate synthase from Rhodospirillum rubrum.”  Baltscheffsky M.et.al.   9630689
[2] “Differential regulation of soluble and membrane-bound inorganic pyrophosphatases in the photosynthetic bacterium Rhodospirillum rubrum provides insights into pyrophosphate-based stress bioenergetics.”  Lopez-Marques R.L.et.al.   15292143
[3] “H+-pyrophosphatase of Rhodospirillum rubrum. High yield expression in Escherichia coli and identification of the Cys residues responsible for inactivation my mersalyl.”  Belogurov G.A.et.al.   11956221

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MAGIYLFVVA AALAALGYGA LTIKTIMAAD AGTARMQEIS GAVQEGASAF LNRQYKTIAV 
61:	VGAVVFVILT ALLGISVGFG FLIGAVCSGI AGYVGMYISV RANVRVAAGA QQGLARGLEL 
121:	AFQSGAVTGM LVAGLALLSV AFYYILLVGI GATGRALIDP LVALGFGASL ISIFARLGGG 
181:	IFTKGADVGA DLVGKVEAGI PEDDPRNPAV IADNVGDNVG DCAGMAADLF ETYAVTVVAT 
241:	MVLASIFFAG VPAMTSMMAY PLAIGGVCIL ASILGTKFVK LGPKNNIMGA LYRGFLVSAG 
301:	ASFVGIILAT AIVPGFGDIQ GANGVLYSGF DLFLCAVIGL LVTGLLIWVT EYYTGTNFRP 
361:	VRSVAKASTT GHGTNVIQGL AISMEATALP ALIICAAIIT TYQLSGLFGI AITVTSMLAL 
421:	AGMVVALDAY GPVTDNAGGI AEMANLPEDV RKTTDALDAV GNTTKAVTKG YAIGSAGLGA 
481:	LVLFAAYTED LAFFKANVDA YPAFAGVDVN FSLSSPYVVV GLFIGGLLPY LFGSMGMTAV 
541:	GRAAGSVVEE VRRQFREIPG IMEGTAKPEY GRCVDMLTKA AIKEMIIPSL LPVLAPIVLY 
601:	FVILGIADKS AAFSALGAML LGVIVTGLFV AISMTAGGGA WDNAKKYIED GHYGGKGSEA 
661:	HKAAVTGDTV GDPYKDTAGP AVNPMIKITN IVALLLLAVL AH