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3.A.12.1.1
Septum DNA translocation pore protein SpoIIIE. SpoIIIE forms DNA-conducting channels across the two lipid bilayers of the septum (Burton et al., 2007). The dynamic assembly of SpoIIIE mediates septal membrane fission during Bacillus subtilis sporulation (Fleming et al., 2010).  Directionality of DNA pumping is probably a result of asymmetic chromosomal distrbution of SpoIIIE Recognition Sequences in the DNA.  Since SpoIIIE can strip proteins off the chromosome during translocation, SpoIIIE may interact with the DNA beyond it's binding sites (Higgins and Dworkin 2012).

Accession Number:P21458
Protein Name:SP3E aka SpoIIIE
Length:787
Molecular Weight:87181.00
Species:Bacillus subtilis [1423]
Number of TMSs:4
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate DNA

Cross database links:

HEGENOM: HBG662140
RefSeq: NP_389562.2   
Entrez Gene ID: 936503   
Pfam: PF09397    PF01580   
KEGG: bsu:BSU16800   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005524 F:ATP binding
GO:0003677 F:DNA binding
GO:0017111 F:nucleoside-triphosphatase activity
GO:0007049 P:cell cycle
GO:0051301 P:cell division
GO:0007059 P:chromosome segregation
GO:0030435 P:sporulation resulting in formation of a cel...

References (8)

[1] “Nucleotide sequence of the sporulation operon, spoIIIE, of Bacillus subtilis.”  Butler P.D.et.al.   3129532
[2] “The role of the sporulation gene spoIIIE in the regulation of prespore-specific gene expression in Bacillus subtilis.”  Foulger D.et.al.   2507870
[3] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377
[4] “From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later.”  Barbe V.et.al.   19383706
[5] “Bacillus subtilis spoIIIE protein required for DNA segregation during asymmetric cell division.”  Wu L.J.et.al.   8160014
[6] “An in vivo membrane fusion assay implicates SpoIIIE in the final stages of engulfment during Bacillus subtilis sporulation.”  Sharp M.D.et.al.   10588743
[7] “Role of Bacillus subtilis SpoIIIE in DNA transport across the mother cell-prespore division septum.”  Bath J.et.al.   11062134
[8] “Role of cell-specific SpoIIIE assembly in polarity of DNA transfer.”  Sharp M.D.et.al.   11778051

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Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MAKKKRKSRK KQAKQLNIKY ELNGLLCIAI SIIAILQLGV VGQTFIYLFR FFAGEWFILC 
61:	LLGLLVLGVS LFWKKKTPSL LTRRKAGLYC IIASILLLSH VQLFKNLTHK GSIESASVVR 
121:	NTWELFLMDM NGSSASPDLG GGMIGALLFA ASHFLFASTG SQIMAIVMIL IGMILVTGRS 
181:	LQETLKKWMS PIGRFIKEQW LAFIDDMKSF KSNMQSSKKT KAPSKKQKPA RKKQQMEPEP 
241:	PDEEGDYETV SPLIHSEPII SSFSDRNEEE ESPVIEKRAE PVSKPLQDIQ PETGDQETVS 
301:	APPMTFTELE NKDYEMPSLD LLADPKHTGQ QADKKNIYEN ARKLERTFQS FGVKAKVTQV 
361:	HLGPAVTKYE VYPDVGVKVS KIVNLSDDLA LALAAKDIRI EAPIPGKSAI GIEVPNAEVA 
421:	MVSLKEVLES KLNDRPDAKL LIGLGRNISG EAVLAELNKM PHLLVAGATG SGKSVCVNGI 
481:	ITSILMRAKP HEVKMMMIDP KMVELNVYNG IPHLLAPVVT DPKKASQALK KVVNEMERRY 
541:	ELFSHTGTRN IEGYNDYIKR ANNEEGAKQP ELPYIVVIVD ELADLMMVAS SDVEDSITRL 
601:	SQMARAAGIH LIIATQRPSV DVITGVIKAN IPSRIAFSVS SQTDSRTILD MGGAEKLLGR 
661:	GDMLFLPVGA NKPVRVQGAF LSDDEVEKVV DHVITQQKAQ YQEEMIPEET TETHSEVTDE 
721:	LYDEAVELIV GMQTASVSML QRRFRIGYTR AARLIDAMEE RGVVGPYEGS KPREVLLSKE 
781:	KYDELSS