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3.A.12.1.2
Cell division protein, FtsK. FtsK lacking its integral membrane domain catalyzes fully efficient chromosome dimer resolution (Dubarry and Barre, 2010).  Its topology has been examined (Berezuk et al. 2014).

Accession Number:P46889
Protein Name:FtsK aka B0890
Length:1329
Molecular Weight:146663.00
Species:Escherichia coli [83333]
Number of TMSs:4
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2 / Cytoplasmic side3
Substrate DNA

Cross database links:

Genevestigator: P46889
EchoBASE: EB3016
EcoGene: EG13226
eggNOG: COG1674
HEGENOM: HBG662140
DIP: DIP-9703N
RefSeq: AP_001520.1    NP_415410.1   
Entrez Gene ID: 945102   
Pfam: PF09397    PF01580   
BioCyc: EcoCyc:G6464-MONOMER    ECOL168927:B0890-MONOMER   
KEGG: ecj:JW0873    eco:b0890   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0005524 F:ATP binding
GO:0015616 F:DNA translocase activity
GO:0005515 F:protein binding
GO:0043565 F:sequence-specific DNA binding
GO:0007049 P:cell cycle
GO:0000920 P:cytokinetic cell separation
GO:0007059 P:chromosome segregation
GO:0045941 P:positive regulation of transcription
GO:0009651 P:response to salt stress
GO:0009432 P:SOS response

References (11)

[1] “A new Escherichia coli cell division gene, ftsK.”  Begg K.J.et.al.   7592387
[2] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Membrane topology of the N-terminus of the Escherichia coli FtsK division protein.”  Dorazi R.et.al.   10922461
[6] “FtsQ, FtsL and FtsI require FtsK, but not FtsN, for co-localization with FtsZ during Escherichia coli cell division.”  Chen J.C.et.al.   11703663
[7] “ZipA is required for recruitment of FtsK, FtsQ, FtsL, and FtsN to the septal ring in Escherichia coli.”  Hale C.A.et.al.   11948172
[8] “FtsK is a DNA motor protein that activates chromosome dimer resolution by switching the catalytic state of the XerC and XerD recombinases.”  Aussel L.et.al.   11832210
[9] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
[10] “Double-stranded DNA translocation: structure and mechanism of hexameric FtsK.”  Massey T.H.et.al.   16916635
[11] “The FtsK gamma domain directs oriented DNA translocation by interacting with KOPS.”  Sivanathan V.et.al.   17057717
Structure:
2IUS   2J5P     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MSQEYIEDKE VTLTKLSSGR RLLEALLILI VLFAVWLMAA LLSFNPSDPS WSQTAWHEPI 
61:	HNLGGMPGAW LADTLFFIFG VMAYTIPVII VGGCWFAWRH QSSDEYIDYF AVSLRIIGVL 
121:	ALILTSCGLA AINADDIWYF ASGGVIGSLL STTLQPLLHS SGGTIALLCV WAAGLTLFTG 
181:	WSWVTIAEKL GGWILNILTF ASNRTRRDDT WVDEDEYEDD EEYEDENHGK QHESRRARIL 
241:	RGALARRKRL AEKFINPMGR QTDAALFSGK RMDDDEEITY TARGVAADPD DVLFSGNRAT 
301:	QPEYDEYDPL LNGAPITEPV AVAAAATTAT QSWAAPVEPV TQTPPVASVD VPPAQPTVAW 
361:	QPVPGPQTGE PVIAPAPEGY PQQSQYAQPA VQYNEPLQQP VQPQQPYYAP AAEQPAQQPY 
421:	YAPAPEQPVA GNAWQAEEQQ STFAPQSTYQ TEQTYQQPAA QEPLYQQPQP VEQQPVVEPE 
481:	PVVEETKPAR PPLYYFEEVE EKRAREREQL AAWYQPIPEP VKEPEPIKSS LKAPSVAAVP 
541:	PVEAAAAVSP LASGVKKATL ATGAAATVAA PVFSLANSGG PRPQVKEGIG PQLPRPKRIR 
601:	VPTRRELASY GIKLPSQRAA EEKAREAQRN QYDSGDQYND DEIDAMQQDE LARQFAQTQQ 
661:	QRYGEQYQHD VPVNAEDADA AAEAELARQF AQTQQQRYSG EQPAGANPFS LDDFEFSPMK 
721:	ALLDDGPHEP LFTPIVEPVQ QPQQPVAPQQ QYQQPQQPVP PQPQYQQPQQ PVAPQPQYQQ 
781:	PQQPVAPQQQ YQQPQQPVAP QQQYQQPQQP VAPQPQDTLL HPLLMRNGDS RPLHKPTTPL 
841:	PSLDLLTPPP SEVEPVDTFA LEQMARLVEA RLADFRIKAD VVNYSPGPVI TRFELNLAPG 
901:	VKAARISNLS RDLARSLSTV AVRVVEVIPG KPYVGLELPN KKRQTVYLRE VLDNAKFRDN 
961:	PSPLTVVLGK DIAGEPVVAD LAKMPHLLVA GTTGSGKSVG VNAMILSMLY KAQPEDVRFI 
1021:	MIDPKMLELS VYEGIPHLLT EVVTDMKDAA NALRWCVNEM ERRYKLMSAL GVRNLAGYNE 
1081:	KIAEADRMMR PIPDPYWKPG DSMDAQHPVL KKEPYIVVLV DEFADLMMTV GKKVEELIAR 
1141:	LAQKARAAGI HLVLATQRPS VDVITGLIKA NIPTRIAFTV SSKIDSRTIL DQAGAESLLG 
1201:	MGDMLYSGPN STLPVRVHGA FVRDQEVHAV VQDWKARGRP QYVDGITSDS ESEGGAGGFD 
1261:	GAEELDPLFD QAVQFVTEKR KASISGVQRQ FRIGYNRAAR IIEQMEAQGI VSEQGHNGNR 
1321:	EVLAPPPFD