TCDB is operated by the Saier Lab Bioinformatics Group

The ATP hydrolysis-dependent TRC receptor TRC40 (Asna-1) (Stefanovic and Hegde, 2007). (TRC40 is homologous to the ArsA ATPase of E. coli (TC# 3.A.4.1.1) and the GET3 ATPase of yeast (TC# 8.A.26.1.1)) Loss yields embryonic lethality. Tryptophan-rich basic protein (WRB) is the tail-anchored (TA) protein insertion receptor. Also called congenital heart disease protein-5 (CHD5). Related to the yeast Get1 protein in 3.A.21.1.1. Calcium-modulating cyclophilin ligand (CAML) is a mammal-specific receptor for TRC40, an ATPase targeting newly synthesized TA proteins.  CAML mediates membrane insertion of TA proteins.  TRC40 (Asna1) has been shown to mediate membrane insertion of two proteins, RAMP4 and Sec61beta, without the participation of other cytosolic proteins by a mechanism that depends on the presence of ATP or ADP and a protease-sensitive receptor in the ER membrane (Favaloro et al. 2010).  TRC40 is required for release of Herpes simplex virus 1 (HSV1) virions (Ott et al. 2016). The functions and reciprocal interactions of the two subunits of the heteromeric TRC40 recpeptor, WBR and CAML (CAMLG), have revealed mutual dependencies for stability; CAML seems to normally be present in 5-fold excess over WBR (Colombo et al. 2016). CAMLG interacts with Classical Swine Fever Virus (CSFV) p7 and mediates calcium permeability in the ER (Gladue et al. 2018).

The TRC complex of Homo sapiens
TRC40 (O43681)
WRB (CHO5) (O00258)
CAML (P49069)

GET1 protein homologue

GET1 of Aspergillus niger (A2QHQ3)

Get3 ATPase homologue of 349 aas and 0 TMSs that drives the insertion of tail anchored (TA) proteins into the endoplasmic reticulum membrane.  The 3-d structure of the tetramer has been solved (Suloway et al. 2012). The tetramer generates a hydrophobic chamber that probably binds the single C-terminal TMS of the TA protein.

Get3 ATPase of Methanocaldococcus jannaschii (Methanococcus jannaschii)

CHD5 homologue

GET1 homologue of Glycine max (I1L4Q8)

CHD5 homologue

GET homologue of Arabdiopsis thaliana (Q1H5D2)