TCDB is operated by the Saier Lab Bioinformatics Group
TCIDNameDomainKingdom/PhylumProtein(s)
*3.A.7.1.1









Conjugal DNA-protein transfer complexes. The VirB4 ATPase may mediate dislocation of the membrane-integrated VirB2 pilin (N- and C-termini out; cytoplasmic loop= residues 90-94 inside) during biogenesis of the Agrobacterium VirB/VirD4 type IV secretion system.

Bacteria
Proteobacteria

VirB complex of Agrobacterium tumefaciens
VirB2 precursor
VirB3
VirB4
VirB5
VirB6
VirB7
VirB8
VirB9
VirB10
VirB11
VirD4
TraG
*3.A.7.2.1









Trw conjugation system of E. coli plasmid IncP. The hexameric form of TrwK is a catalytically active ATPase, much like the structurally related protein TrwB, the conjugative coupling protein (Arechaga et al, 2008), which is a structure-specific DNA-binding protein (Matilla et al., 2010)

Bacteria
Proteobacteria

Trw conjugation system of E. coli plasmid R388
TrwL1 (like VirB2)
TrwM (like VirB3)
TrwK (like VirB4)
TrwJ (like VirB5)
TrwI (like VirB6)
TrwH (like VirB7)
TrwG (like VirB8)
TrwF (like VirB9)
TrwE (like VirB10)
TrwD (like VirB11)
TrwB (like TraG and VirD4)
*3.A.7.3.1









Pertussis toxin exporter. For consideration of the requirements for assembly, see Verma and Burns, 2007.
Bacteria
Proteobacteria

Pertussis toxin exporter of Bordetella pertussis
PtlA (like VirB2)
PtlB (like VirB3)
PtlC (like VirB4)
PtlD (like VirB6)
PtlI (like VirB7)
PtlE (like VirB8)
PtlF (like VirB9)
PtlG (like VirB10)
PtlH (like VirB11)
*3.A.7.4.1









Trb proteins of Enterobacter aerogenes plasmid IncP
Bacteria
Proteobacteria
Trb proteins of Enterobacter aerogenes plasmid IncP
TrbC (like VirB2)
TrbD (like VirB3)
TrbE (like VirB4)
TrbJ (like VirB5)
TrbL (like VirB6)
TrbK (like VirB7)
TrbF (like VirB8)
TrbG (like VirB9)
TrbI (like VirB10)
TrbB (like VirB11)
*3.A.7.4.2









P-type conjugal transfer system

Bacteria
Fusobacteria
Conjugal transfer system of Ilyobacter polytropus 
TraG (620 aas; E3HE20)
TrbB (310 aas; E3HE22)
TrbC (106 aas; E3HE16)
TrbD (85 aas; E3HE23)
TrbE (804 aas; E3HE24)
TrbF (227 aas; E3HE17) 
TrbG (274 aas; E3HE18)
TrbI (364 aas; E3HE19)
TrbJ (251 aas; E3HE25)
TrbL (350 aas; E3HE26)
Uncharacterized protein (117 aas; E3HE21) 
*3.A.7.5.1









LvhB proteins of Legionella pneumophila (De Buck et al., 2007)

Bacteria
Proteobacteria
LvhB proteins of Legionella pneumophila
LvhB2 (like VirB2)
LvhB3 (like VirB3)
LvhB4 (like VirB4)
LvhB5 (like VirB5)
LvhB6 (like VirB6)
LvhB7 (like VirB7)
LvhB8 (like VirB8)
LvhB9 (like VirB9)
LvhB10 (like VirB10)
LvhB11(like VirB11)
LvhD4 (like VirD4) (Q5ZW42)
*3.A.7.6.1









Tra proteins of plasmid Inc N (Escherichia coli)
Bacteria
Proteobacteria
Tra proteins of plasmid Inc N (Escherichia coli)
TraM (like VirB2)
TraA (like VirB3)
TraB (like VirB4)
TraC (like VirB5)
TraD (like VirB6)
TraN (like VirB7)
TraE (like VirB8)
TraO (like VirB9)
TraF (like VirB10)
TraG (like VirB11)
*3.A.7.7.1









Trs DNA transfer protein complex of plasmid pGO1 of Staphylococcus aureus (Grohman et al., 2003)
Bacteria
Firmicutes
Trs proteins of Staphylococcus aureus plasmid pGO1
TrsA (B36891)
TrsB (C36891)
TrsC (D36891)
TrsD (E36891)
TrsE (F36891)
TrsF (G36891)
TrsG (H36891)
TrsH (I36891)
TrsI (A56976)
TrsJ (B56976)
TrsK (C56976)
TrsL (D56976)
TrsM (E56976)
TrsN (A36891)
TrsO (F56976)
*3.A.7.8.1









Competence system for DNA uptake (ComB) (Hofreuter et al., 2003; Karnholz et al., 2006). DNA uptake into the periplasm occurs preferentially at the poles. Transport into the cytoplasm depends on ComEC. Thus, DNA uptake may occur in a two step process (Stingl et al., 2010).

Bacteria
Proteobacteria
ComB1-4 and ComB6-10 of Helicobacter pylori
ComB1 (CAA10655)
ComB2 (CAA10656)
ComB3 (CAA10657)
ComB4 (AAS03033)
ComB6 (hp0037; Hp26695 genome)
ComB7 (hp0038; Hp26695 genome)
ComB8 (AAM03034)
ComB9 (AAM03035)
ComB10 (AAM03036)
*3.A.7.9.1









The Icm/Dot protein secretion system. IcmS and IcmW form a complex that interacts with and may translocate substrate proteins (Ninio et al., 2005; De Buck et al., 2007; Cambronne and Roy, 2007). The crystal structure of the IcmR-IcmQ complex has been solved (Raychaudhury et al., 2009).

Bacteria
Proteobacteria
The Icm/Dot system of Legionella pneumophila
IcmT1 (IM; like TraK) (AAU26538)
IcmS (Cyt) (AAU26539)
IcmR (Cyt) (AAU26540)
IcmQ (Cyt) (AAU26541)
IcmP/DotM (L Trb) (IM; like TrbA) (AAU26542)
IcmO/DotL (IM; like TrbC and VirD4) (AAU26543)
IcmN/DotK/LphA (peri) (AAU26544)
IcmM/DotJ (IM) (AAU26545)
IcmL/DotI (IM; like TraM) (AAU26546)
IcmK/DotH (peri; like TraN) (AAU26547)
IcmE/DotG (IM; like TraO and VirB10) (AAU26548)
IcmG/DotF (IM; like TraP) (AAU26549)
IcmC/DotE (IM; like TraQ) (AAU26550)
IcmD/DotP (IM; like TraR) (AAU26551)
IcmJ/DotN (Cyt; like TraT) (AAU26552)
IcmB/DotB (Cyt; like TraU and VirB4) (AAU26553)
IcmF (IM) (AAU26554)
IcmH (IM) (AAU26555)
IcmW (Cyt) (AAU28746)
IcmX (peri; like TraW) (AAU28747)
IcmV (IM; like TraX) (AAU28745)
DotA (IM; like TraY) (AAK63144)
DotB (Cyt; like TraJ and VirB11) (AAU28734)
DotC (peri; like TraI) (AAU28733)
DotD (peri; like TraH) (AAU28732)
*3.A.7.10.1









The E. coli plasmid R64 IncI conjugation system
other sequences
plasmids
The IncI conjugation system of plasmid R64 of E. coli
TrbA (BAA78018)
TrbB (BAA78019)
TrbC (BAA78020)
TraH (BAA77993)
TraI (BAA77994)
TraJ (BAA77995)
TraK (BAA77996)
TraL (BAA78000)
TraM (BAA78001)
TraN (BAA78002)
TraO (BAA78003)
TraP (BAA78004)
TraQ (BAA78005)
TraR (BAA78006)
TraS (BAA78007)
TraT (BAA78008)
TraU (BAA78009)
TraV (BAA78010)
TraW (BAA78011)
TraX (BAA78012)
TraY (BAA78013)
*3.A.7.11.1









The type IV β-proteobacterial DNA secretion system (non contact dependent) for natural transformation)

Bacteria
Proteobacteria
DNA secretion system of Neisseria gonorrhoeae
TraD (Q5EPC8)
TraI (Q5EPC7)
TraA (Q5EPC3)
TraL (Q5EPC2)
TraE (Q5EPC1)
TraK (Q5EPC0)
TraB (Q5EPB9)
TraV (Q5EPB7)
TraC (Q5EPB6)
TrbI (Q5EPB4)
TraW (Q5EPB3)
TraU (Q5EPB2)
TrbC (Q5EPB1)
TraN (Q5EPA9)
TraF (Q5EPA7)
TraH (Q93NF2)
TraG (Q5EPA5)
DsbC (Q5K698)
AtlA (O30554)
ParA (Q5EP68)
*3.A.7.12.1









The Cag pathogenicity island (PAI) proteins (Kutter et al., 2008).  CagD (Cag24) is essential for CagA translocation and maximal induction of interleukin-8 secretion (Cendron et al., 2009). It is required for a pro-inflammatory response and multiple cytoskeletal and gene regulatory effects in gastric epithelial cells. Its molecular properties are described in a review by Fischer (2011). Cag16 (CagM) (of unknown function and not dermonstrably homologous to proteins of type IV secretion systems in other bacteria) has 3-4 TMSs and is essential for CagA export. It may therefore be a core protein of the Cag complex (Ling et al. 2013).

Bacteria
Proteobacteria
The Cag PAI proteins of Helicobacter pylori:
Cag1 O25257
Cag2 ABO28051
Cag3 O25258
Cag4 (like VirD4) O25259
Cag5 O25260
Cag6 (like VirB10) O25261
Cag7 (like VirB9) O25262
Cag8 O25263
Cag9 (like VirB4) O25264
Cag10 O25265
Cag11 O25266
Cag12 P97245
Cag13 P97227
Cag14 O25268
Cag15 O25269
Cag16 O25270
Cag17 O25271
Cag18 O25272
Cag19 O25273
Cag20 O25274
Cag21 O25275
Cag22 O25276
Cag23 Q48252
Cag24 O25277
Cag25 O25278
*3.A.7.13.1









The putative type IV secretion system of Bacillus anthracis (most genes are encoded on the two virulience plasmids pXO1 and pXO2; Grynberg et al., 2007).

Bacteria
Firmicutes
Type IV secretion system of Bacillus anthracis
pXO1-42 (like VirD4; BXA0064; Q8KYQ0)
pXO1-57 (like VB4; BXA0083; Q8KYP1)
pXO1-58 (like CpaE; BXA0084; Q8KYP0)
pXO1-59 (like CpaF, VirB11; BXA0085; Q7CMH0)
pXO1-60 (like TadB, TadC; BXA0086; Q7CMG9)
pXO1-61 (like TadB, TadC; BXA0087; Q8KYN9)
pXO1-63 (like PilA; BXA0089; Q8KYN8)
pXO1-64 (like PilA; BXA0090; Q7CMG7)
pXO1-66 (like PapD; BXA0092; Q7CMG6)
pXO1-67 (like CheW; BXA0093; Q7CMG5)
pXO1-68 (like CpaA; BXA0068; Q8KYP9)
pXO1-77 (like VirB4; BXA0077; Q7CMH3)
pXO1-78 (like VB4; BXA0107; Q8R8B3)
pXO1-79 (like VB6; BXA0108; Q7CMF7)
pXO2-09 (like VirB4-C; BXB0008; Q6F059)
pXO2-10 (like VirB4-N; BXB0009; Q9RN22)
pXO2-15 (like VirD4-C; BXB0014; Q9RN17)
pXO2-16 (like VirD4-N; BXB0015; Q7CMB1)
pXO2-23/24 (like TadB, TadC; BXB0022; Q9RN08)
pXO2-25 (like CpaF, TadA, VirB11; BXB0023; Q6F054)
BA4072 (like VD4; Q81WB0)
*3.A.7.13.2









The plasmid pLS20 conjugation system. Several conserved conjugation proteins carried on the Bacillus subtilis plasmid pLS20, (VirB1, VirB4, VirB11, VirD2, and VirD4 homologs) assemble at a single cell pole, but also at other sites along the cell membrane, in cells during the lag phase of growth (Bauer et al., 2011).

Bacteria
Firmicutes
Plasmid pLS20 conjugation system of Bacillus subtilis 
VirB1 (Orf30) (E9RJ18)
VirB4 (Orf24) (E9RJ16)
VirB11 (Orf13) (E9RJ00)
VirD2 (Orf34) (E9RJ23)
VirD4 (Orf24) (E9RJ12) 
PO16 (E9RJ01)
PO17 (E9RJ02)
PO25 (E9RJ10)
PO26 (E9RJ11)
PO27 (E9RJ12)
PO28 (E9RJ13)
PO32 (E9RJ17)
PO34 (E9RJ19) 
*3.A.7.14.1









Type 4 conjugal transfer (tra) system of Gram-positive bacteria, Orf1-Orf15 (Abajy et al., 2007).
other sequences
plasmids
Orf1-Orf15 of Gram-positive bacterial (Enterococcus faecalis) conjugal plasmid, pIP501 (most resembles the Trs system of S. aureus; TC#3.A.7.7.1).
Orf1 (654aas) (Q52001) (A relaxase, resembles EsaA of S. aureus, 9.A.44.1.1 and MLP1 of S. cerevisiae, 9.A.14.1.1)
Orf2 (110aas) (Q52002) (homologous to TrsB of S. aureus)
Orf3 (127aas) (Q52003) (homologous to TrsC of S. aureus)
Orf4 (190aas) (Q52004) (homologous to TrsD of S. aureus)
Orf5 (653aas) (Q52005) (VirB4-like ATPase; homologous to TrsE of S. aureus)
Orf6 (450aas) (Q52006) (homologous to TrsF of S. aureus)
Orf7 (369aas) (Q8L1D1) (lytic transglycosidase; like TrsG)
Orf8 (183aas) (Q9AL12) (like TrsA)
Orf9 (143aas) (Q9AL11)
Orf10 (551aas) (Q8L1D0) (VirD4-like putative coupling protein; like TrsK)
Orf11 (307aas) (Q8L1C9) (most similar to Streptolysin O (1.C.12.1.4))
Orf12 (310aas) (Q8L1C8) (like TrsL)
Orf13 (322aas) (Q8L1C7)
Orf14 (122aas) (Q7BVV5)
Orf15 (282aas) (Q8L1C6) (contains a long stretch TEP tripeptide repeats)
*3.A.7.14.2









Conjugation system, IntP/TcpA-J (Bannam et al., 2008; Teng et al., 2008). TcpA but not TcpB is essential for transfer of the conjugative plasmid pCW3 (Parsons et al., 2007). TcpA interacts with other pCW3-encoded proteins to form an essential part of the conjugation complex (Steen et al., 2009).

Bacteria
Firmicutes
IntP/TcpA-J of Clostridium perfringens
IntP (251aas;0TMSs) (Q15HV4)
TcpA (532aas;3TMSs) (Shows similarity to proteins in 3.A.12; e.g. SpoIIIE) (Q15HV3)
TcpB (302aas;0TMSs) (Shows similarity to proteins in 3.A.12; e.g. SpoIIIE) (Q2L5Y2)
TcpC (359aas;1TMS) (Q15HV1)
TcpD (115aas;3TMSs) (Q15HV0)
TcpE (125aas;2TMSs) (Q15HU9)
TcpF (840aas;0TMSs) (shows limited sequence similarity to 3.A.7.13.1 Q8R8B3) (Q15HU7)
TcpG (334aas;1TMS) (Q15HU6)
TcpH (831aas;8TMSs(4+4)) (Contains UirB6-like domains and shows sequence similarity in the same regions to 3.A.1.14.18 (Q5HGV2) and 3.A.7.12.1 (O25262)) (Q15HU5)
TcpI (164aas;4TMSs) (Q15HU4)
TcpH (258aas;1TMS) (Q15HU3)
*3.A.7.15.1









The nonspecific tight adherence fibril (pilus) secretion septum (Flp-1, Flp-2, TadV-RcpCAB Tad ZA-G) (Kachlany et al., 2000; Bhattacharjee et al., 2001; Haase et al., 2003; Schreiner et al., 2003; Wang and Chen, 2005; Tomich et al. 2007).

Bacteria
Proteobacteria
Flp-1,2;
RcpCAB;
TadA-G, V, Z of Acinobacillus (Aggregatibacter) actinomycetemcomitans
Flp-1 (75aas) (Q9ANW5)
Flp-2 (76aas) (O66150)
RcpA secretin (460aas) (Q8GD00)
RcpB (167aas) (Q9X6J2)
RcpC (shows 25% identity, 41% similarity with 3.A.1.122.2; O31712)(274aas) (Q8GD01)
TadA (ATPase; Most like LvhB11; 3.A.7.5.1; Q9RLR3) (426aas) (Q9XC06)
TadB (295aas) (Q9XC05)
TadC (288aas) (Q9S4A8)
TadD (Most like 8.A.11.1.1; Q9LDC0 peptidyl prolyl isomerase) (253aas) (Q9S4A7)
TadE (191aas) (Q9S4A6)
TadF (Most like 3.A.7.14.1; Q52001) (200aas) (Q9S4A5)
TadG (Most similar to 1.A.13.1.2; Q9UNF7; Ca2+-activated Cl- channel)
(Q9S4A4) TadV (142aas) (Q8GD02)
TadZ (374aas) (Q8GCZ9)
*3.A.7.16.1









F-like conjugation DNA transfer system (Greub et al., 2004).

Bacteria
Chlamydiae/Verrucomicrobia group
The F-type conjugation system of Protochlamydia amoebophila
TraA; TrbB; RecD (721aas) (Q6MDD6)
TrbC (198aas) (Q6MB92) 
TraB (408aas) (Q6MBA0)
TraC (828aas) (Q6MB95)
TraD (556aas) (Q6MB84)
TraE (189aas) (Q6MBA2) 
TraFa (160aas) (Q6MB94)
TraFb (263aas) (Q6MB87)
TraG (927aas) (Q6MB85)
TraH (452aas) (Q6MB86)
TraN (184aas) (Q6MB88)
TraU (311aas) (Q6MB91)
TraW (210aas) (Q6MB93) 
*3.A.7.17.1









The EsxA/B protein secretion pathway system (EPSP) (Anderson et al. 2011; Burts et al. 2005; Chen et al. 2012; Sundaramoorthy et al. 2008).  EssC is an integral component of the bacterial ""Type VII"" secretion system (T7SS). The protein is predicted to consist of an intracellular repeat of forkhead-associated (FHA) domains at the N-terminus, two transmembrane helices and three P-loop containing ATPase-type domains, D1-3, forming the C-terminal intracellular segment. Zoltner et al. 2016 presented crystal structures of the N-terminal FHA domains (EssC-N) and a C-terminal fragment EssC-C from Geobacillus thermodenitrificans, encompassing two of the ATPase-type modules, D2 and D3. Module D2 binds ATP with high affinity whereas D3 does not. The EssC-N and EssC-C constructs are monomeric in solution but the full-length recombinant protein, with molecular weight about 169 kDa, forms a multimer of approximately 1 MDa. The observation of protomer contacts in the crystal structure of EssC-C together with similarity to the DNA translocase FtsK, suggests a model for a hexameric EssC assembly. Such an observation potentially identifies the key component of pore formation required for secretion. The extreme C-terminal ATPase domain appears essential for EssC activity as a key part of the T7SS whilst D2 and FHA domains are required for the production of a stable and functional protein (Zoltner et al. 2016). TheTMSs of EssA and EssB interact, regulating secretion (Ahmed et al. 2018). This system resembles the ESX systems of Mycobacterium tuberculosis (TC# 3.A.24).

 

Bacteria
Firmicutes
EPSP of Staphylococcus aureus (EsaA-C/EssA-C)
EsaA (1009 aas) (Q99WU3)
EsaB (80 aas) (Q99WU1)
EsaC (130 aas) (Q99WT8)
EssA (152 aas) (Q99WU2)
EssB (444 aas) (Q99WU0)
EssC (1479 aas) (Q932J9)
EsaD (617aas) (A6QDR8) 
*3.A.7.18.1









The Cpa protein secretion system, PilA-CpaA-F. PilA (58aas) is similar to Flp pilin (1.E.52.3.2) (Tomich et al. 2007).

Bacteria
Proteobacteria
The Cpa system of Caulobacter crescentus
PilA (pilin) (Q9L720)
CpaA (Prepilin peptidase) (Q9L719)
CpaB (Pilus assembly protein) (Q9L718)
CpaC Secretin (Q9L717)
CpaD (Pilus assembly protein) (Q9L716)
CpaE (Pilus assembly ATPase) (Q9L715)
CpaF (Pilus assembly ATPase) (Q9L714)
*3.A.7.19.1









The putative Pcf type IV protein/DNA secretion (conjugation) system of the Enterococcus faecalis plasmid pCF10. All Pcf proteins with more than 100 aas were included (Hirt et al. 2005).

Bacteria
Firmicutes
The Pfc system of Enterococcus faecalis pCF10 plasmid.  All Pcf proteins with more than 100 aas were included (Hirt et al. 2005).
PcfY, 206 aas, Q5G3L7
PcfC, 609 aas, 3 TMSs, Q5G3N9
PcfD, 719 aas, Q5G3N8
PcfG, 561 aas, Q7X5P3
PcfK, 258 aas, Q5G3N1
PcfJ, 445 aas, Q5G3N2
PcfV, 341 aas, 10 TMSs, Q5G3M0
PcfB, 161 aas, Q5G3P0
PcfF, 118 aas, Q5G3N6
PcfO, 149 aas, 1 TMS, Q5G3M7
PcfA, 202 aas, Q5G3P1
PcfX, 219 aas, Q5G3L8
PcfR, 164 aas, 2 TMSs, Q5G3M4
PcfU, 109 aas, Q5G3M1
PcfL, 182 aas, Q5G3N0
PcfH, 116 aas, 3 TMSs, Q5G3N4
PcfS, 150 aas, ssDNA binding protein, Q5G3M3
PcfT, 192 aas, Q5G3M2