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3.E.1.2.1
Halorhodopsin Cl- uptake pump; homologous to bacteriorhodopsin (3.E.1.1.1) which can be converted from a proton pump with outwardly directed polarity into a chloride pump with inwardly directed polarity via a single amino acid substitution at position 85.  Cl- transport does not depend on water hydrogen bonded to the chromophore as in the case of bacteriorhodopsin (Muroda et al. 2012).  However, inter-helical hydrogen bonds, mediated by a key arginine residue, largely govern the dynamics of the protein and water groups coordinating the chloride ion (Jardón-Valadez et al. 2014).  Helices E and F probably move considerable during chloride binding and ion transport (Schreiner et al. 2016).

Accession Number:P16102
Protein Name:BACH aka HOP aka VNG0180G
Length:274
Molecular Weight:28874.00
Species:Halobacterium salinarium [2242]
Number of TMSs:7
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Cl-

Cross database links:

HEGENOM: HBG497864
RefSeq: NP_279315.1   
Entrez Gene ID: 1447103   
Pfam: PF01036   
BioCyc: HSP64091:VNG0180G-MONOMER   
KEGG: hal:VNG0180G   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005216 F:ion channel activity
GO:0009881 F:photoreceptor activity
GO:0006811 P:ion transport
GO:0007602 P:phototransduction
GO:0018298 P:protein-chromophore linkage

References (3)

[1] “The halo-opsin gene. II. Sequence, primary structure of halorhodopsin and comparison with bacteriorhodopsin.”  Blanck A.et.al.   15981336
[2] “Genome sequence of Halobacterium species NRC-1.”  Ng W.V.et.al.   11016950
[3] “Structure of the light-driven chloride pump halorhodopsin at 1.8-A resolution.”  Kolbe M.et.al.   10827943
Structure:
1E12   2JAF   2JAG   5ahy     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSITSVPGVV DAGVLGAQSA AAVRENALLS SSLWVNVALA GIAILVFVYM GRTIRPGRPR 
61:	LIWGATLMIP LVSISSYLGL LSGLTVGMIE MPAGHALAGE MVRSQWGRYL TWALSTPMIL 
121:	LALGLLADVD LGSLFTVIAA DIGMCVTGLA AAMTTSALLF RWAFYAISCA FFVVVLSALV 
181:	TDWAASASSA GTAEIFDTLR VLTVVLWLGY PIVWAVGVEG LALVQSVGVT SWAYSVLDVF 
241:	AKYVFAFILL RWVANNERTV AVAGQTLGTM SSDD