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4.A.1.1.2
N-Acetyl glucosamine (NAG) porter (NagE) (Plumbridge 2015).

Accession Number:P09323
Protein Name:PTAA aka NAGE aka PSTN aka B0679
Length:648
Molecular Weight:68347.00
Species:Escherichia coli [83333]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate N-acetylglucosamine

Cross database links:

Genevestigator: P09323
EchoBASE: EB0629
EcoGene: EG10635
eggNOG: COG1263
HEGENOM: HBG571563
RefSeq: AP_001317.1    NP_415205.1   
Entrez Gene ID: 945292   
Pfam: PF00358    PF00367    PF02378   
BioCyc: EcoCyc:NAGE-MONOMER    ECOL168927:B0679-MONOMER   
KEGG: ecj:JW0665    eco:b0679   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0019866 C:organelle inner membrane
GO:0005886 C:plasma membrane
GO:0016301 F:kinase activity
GO:0008982 F:protein-N(PI)-phosphohistidine-sugar phosph...
GO:0005351 F:sugar:hydrogen symporter activity
GO:0009401 P:phosphoenolpyruvate-dependent sugar phospho...

References (6)

[1] “Nucleotide sequences of the Escherichia coli nagE and nagB genes: the structural genes for the N-acetylglucosamine transport protein of the bacterial phosphoenolpyruvate: sugar phosphotransferase system and for glucosamine-6-phosphate deaminase.”  Rogers M.J.et.al.   3284790
[2] “Sequence of cloned enzyme IIN-acetylglucosamine of the phosphoenolpyruvate:N-acetylglucosamine phosphotransferase system of Escherichia coli.”  Peri K.G.et.al.   3056518
[3] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[4] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[5] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[6] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
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FASTA formatted sequence
1:	MNILGFFQRL GRALQLPIAV LPVAALLLRF GQPDLLNVAF IAQAGGAIFD NLALIFAIGV 
61:	ASSWSKDSAG AAALAGAVGY FVLTKAMVTI NPEINMGVLA GIITGLVGGA AYNRWSDIKL 
121:	PDFLSFFGGK RFVPIATGFF CLVLAAIFGY VWPPVQHAIH AGGEWIVSAG ALGSGIFGFI 
181:	NRLLIPTGLH QVLNTIAWFQ IGEFTNAAGT VFHGDINRFY AGDGTAGMFM SGFFPIMMFG 
241:	LPGAALAMYF AAPKERRPMV GGMLLSVAVT AFLTGVTEPL EFLFMFLAPL LYLLHALLTG 
301:	ISLFVATLLG IHAGFSFSAG AIDYALMYNL PAASQNVWML LVMGVIFFAI YFVVFSLVIR 
361:	MFNLKTPGRE DKEDEIVTEE ANSNTEEGLT QLATNYIAAV GGTDNLKAID ACITRLRLTV 
421:	ADSARVNDTM CKRLGASGVV KLNKQTIQVI VGAKAESIGD AMKKVVARGP VAAASAEATP 
481:	ATAAPVAKPQ AVPNAVSIAE LVSPITGDVV ALDQVPDEAF ASKAVGDGVA VKPTDKIVVS 
541:	PAAGTIVKIF NTNHAFCLET EKGAEIVVHM GIDTVALEGK GFKRLVEEGA QVSAGQPILE 
601:	MDLDYLNANA RSMISPVVCS NIDDFSGLII KAQGHIVAGQ TPLYEIKK