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4.C.1.1.14
Long chain fatty acyl-CoA ligase (synthetase) (E.C. 6.2.1.3) (Pulsifer et al., 2012).

Accession Number:Q9XIA9
Protein Name:Long chain acyl-CoA synthetase 2
Length:665
Molecular Weight:74389.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Location1 / Topology2 / Orientation3: Endoplasmic reticulum1
Substrate fatty acids

Cross database links:

Genevestigator: Q9XIA9
eggNOG: COG1022
HEGENOM: HBG721674
Entrez Gene ID: 841367   
Pfam: PF00501   
KEGG: ath:AT1G49430   

Gene Ontology

GO:0005783 C:endoplasmic reticulum
GO:0009506 C:plasmodesma
GO:0005524 F:ATP binding
GO:0031957 F:very long-chain fatty acid-CoA ligase activity
GO:0010143 P:cutin biosynthetic process
GO:0050832 P:defense response to fungus
GO:0010311 P:lateral root formation
GO:0010025 P:wax biosynthetic process

References (12)

[1] “Arabidopsis contains nine long-chain acyl-coenzyme A synthetase genes that participate in fatty acid and glycerolipid metabolism.”  Shockey J.M.et.al.   12177484
[2] “Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.”  Theologis A.et.al.   11130712
[3] “Empirical analysis of transcriptional activity in the Arabidopsis genome.”  Yamada K.et.al.   14593172
[4] “Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases.”  Shockey J.M.et.al.   12805634
[5] “The acyl-CoA synthetase encoded by LACS2 is essential for normal cuticle development in Arabidopsis.”  Schnurr J.et.al.   14973169
[6] “Osmotic regulation of root system architecture.”  Deak K.I.et.al.   15960613
[7] “A permeable cuticle in Arabidopsis leads to a strong resistance to Botrytis cinerea.”  Bessire M.et.al.   17396154
[8] “The transcription factor WIN1/SHN1 regulates Cutin biosynthesis in Arabidopsis thaliana.”  Kannangara R.et.al.   17449808
[9] “Mutations in LACS2, a long-chain acyl-coenzyme A synthetase, enhance susceptibility to avirulent Pseudomonas syringae but confer resistance to Botrytis cinerea in Arabidopsis.”  Tang D.et.al.   17434992
[10] “Root system architecture in Arabidopsis grown in culture is regulated by sucrose uptake in the aerial tissues.”  Macgregor D.R.et.al.   18952782
[11] “Arabidopsis CER8 encodes LONG-CHAIN ACYL-COA SYNTHETASE 1 (LACS1) that has overlapping functions with LACS2 in plant wax and cutin synthesis.”  Lue S.et.al.   19392700
[12] “Organ fusion and defective cuticle function in a lacs1 lacs2 double mutant of Arabidopsis.”  Weng H.et.al.   20237894

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSLAADNVLL VEEGRPATAE HPSAGPVYRC KYAKDGLLDL PTDIDSPWQF FSEAVKKYPN 
61:	EQMLGQRVTT DSKVGPYTWI TYKEAHDAAI RIGSAIRSRG VDPGHCCGIY GANCPEWIIA 
121:	MEACMSQGIT YVPLYDSLGV NAVEFIINHA EVSLVFVQEK TVSSILSCQK GCSSNLKTIV 
181:	SFGEVSSTQK EEAKNQCVSL FSWNEFSLMG NLDEANLPRK RKTDICTIMY TSGTTGEPKG 
241:	VILNNAAISV QVLSIDKMLE VTDRSCDTSD VFFSYLPLAH CYDQVMEIYF LSRGSSVGYW 
301:	RGDIRYLMDD VQALKPTVFC GVPRVYDKLY AGIMQKISAS GLIRKKLFDF AYNYKLGNMR 
361:	KGFSQEEASP RLDRLMFDKI KEALGGRAHM LLSGAAPLPR HVEEFLRIIP ASNLSQGYGL 
421:	TESCGGSFTT LAGVFSMVGT VGVPMPTVEA RLVSVPEMGY DAFSADVPRG EICLRGNSMF 
481:	SGYHKRQDLT DQVLIDGWFH TGDIGEWQED GSMKIIDRKK NIFKLSQGEY VAVENLENTY 
541:	SRCPLIAQIW VYGNSFESFL VGVVVPDRKA IEDWAKLNYQ SPNDFESLCQ NLKAQKYFLD 
601:	ELNSTAKQYQ LKGFEMLKAI HLEPNPFDIE RDLITPTFKL KRPQLLQHYK GIVDQLYSEA 
661:	KRSMA