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4.C.1.1.4
Long chain fatty acyl CoA synthase (ligase), (EC 6.2.1.3)

Accession Number:P69451
Protein Name:Long-chain-fatty-acid--CoA ligase LCFA aka FadD aka OLDD aka B1805
Length:561
Molecular Weight:62332.00
Species:Escherichia coli [83333]
Number of TMSs:2
Location1 / Topology2 / Orientation3: Membrane1 / Peripheral membrane protein2
Substrate fatty acids

Cross database links:

Genevestigator: P69451 P69451
EchoBASE: EB1492 EB1492
EcoGene: EG11530 EG11530
eggNOG: COG0318 COG0318
HEGENOM: HBG547964 HBG547964
RefSeq: AP_002424.1    NP_416319.1   
Entrez Gene ID: 946327   
Pfam: PF00501    PF13193   
BioCyc: EcoCyc:ACYLCOASYN-MONOMER    ECOL168927:B1805-MONOMER    MetaCyc:ACYLCOASYN-MONOMER   
KEGG: ecj:JW1794    eco:b1805    eco:b1805   

Gene Ontology

GO:0005829 C:cytosol
GO:0009898 C:internal side of plasma membrane
GO:0005524 F:ATP binding
GO:0004467 F:long-chain-fatty-acid-CoA ligase activity
GO:0070538 F:oleic acid binding
GO:0006637 P:acyl-CoA metabolic process
GO:0006635 P:fatty acid beta-oxidation
GO:0008654 P:phospholipid biosynthetic process
GO:0004467 F:long-chain fatty acid-CoA ligase activity

References (12)

[1] “The fadD gene of Escherichia coli K12 is located close to rnd at 39.6 min of the chromosomal map and is a new member of the AMP-binding protein family.”  Fulda M.et.al.   8107670
[2] “Cloning, sequencing, and expression of the fadD gene of Escherichia coli encoding acyl coenzyme A synthetase.”  Black P.N.et.al.   1460045
[3] “A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.”  Itoh T.et.al.   9097040
[4] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[5] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[6] “Functional role of fatty acyl-coenzyme A synthetase in the transmembrane movement and activation of exogenous long-chain fatty acids. Amino acid residues within the ATP/AMP signature motif of Escherichia coli FadD are required for enzyme activity and fatty acid transport.”  Weimar J.D.et.al.   12034706
[7] “The fadD gene of Escherichia coli K12 is located close to rnd at 39.6 min of the chromosomal map and is a new member of the AMP-binding protein family.”  Fulda M.et.al.   8107670
[8] “Cloning, sequencing, and expression of the fadD gene of Escherichia coli encoding acyl coenzyme A synthetase.”  Black P.N.et.al.   1460045
[9] “A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.”  Itoh T.et.al.   9097040
[10] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[11] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[12] “Functional role of fatty acyl-coenzyme A synthetase in the transmembrane movement and activation of exogenous long-chain fatty acids. Amino acid residues within the ATP/AMP signature motif of Escherichia coli FadD are required for enzyme activity and fatty acid transport.”  Weimar J.D.et.al.   12034706

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKKVWLNRYP ADVPTEINPD RYQSLVDMFE QSVARYADQP AFVNMGEVMT FRKLEERSRA 
61:	FAAYLQQGLG LKKGDRVALM MPNLLQYPVA LFGILRAGMI VVNVNPLYTP RELEHQLNDS 
121:	GASAIVIVSN FAHTLEKVVD KTAVQHVILT RMGDQLSTAK GTVVNFVVKY IKRLVPKYHL 
181:	PDAISFRSAL HNGYRMQYVK PELVPEDLAF LQYTGGTTGV AKGAMLTHRN MLANLEQVNA 
241:	TYGPLLHPGK ELVVTALPLY HIFALTINCL LFIELGGQNL LITNPRDIPG LVKELAKYPF 
301:	TAITGVNTLF NALLNNKEFQ QLDFSSLHLS AGGGMPVQQV VAERWVKLTG QYLLEGYGLT 
361:	ECAPLVSVNP YDIDYHSGSI GLPVPSTEAK LVDDDDNEVP PGQPGELCVK GPQVMLGYWQ 
421:	RPDATDEIIK NGWLHTGDIA VMDEEGFLRI VDRKKDMILV SGFNVYPNEI EDVVMQHPGV 
481:	QEVAAVGVPS GSSGEAVKIF VVKKDPSLTE ESLVTFCRRQ LTGYKVPKLV EFRDELPKSN 
541:	VGKILRRELR DEARGKVDNK A