TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


5.A.2.1.1
Disulfide bond formation protein (disulfide oxidoreductase), DsbB

Accession Number:P0A6M2
Protein Name:Disulfide bond formation protein B aka DsbB aka ROXB aka B1185
Length:176
Molecular Weight:20142.00
Species:Escherichia coli [83333]
Number of TMSs:4
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Electrons

Cross database links:

Genevestigator: P0A6M2
EchoBASE: EB1366
EcoGene: EG11393
eggNOG: COG1495
HEGENOM: HBG637909
RefSeq: AP_001810.1    NP_415703.3   
Entrez Gene ID: 946344   
Pfam: PF02600   
Drugbank: Drugbank Link   
BioCyc: EcoCyc:DSBBPROT-MONOMER    ECOL168927:B1185-MONOMER   
KEGG: ecj:JW5182    eco:b1185   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0009055 F:electron carrier activity
GO:0005515 F:protein binding
GO:0015035 F:protein disulfide oxidoreductase activity
GO:0022900 P:electron transport chain
GO:0006810 P:transport

References (9)

[1] “A pathway for disulfide bond formation in vivo.”  Bardwell J.C.A.et.al.   8430071
[2] “Identification and characterization of the Escherichia coli gene dsbB, whose product is involved in the formation of disulfide bonds in vivo.”  Missiakas D.et.al.   7688471
[3] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[4] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[5] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[6] “Cloning, sequencing, and expression of the nhaB gene, encoding a Na+/H+ antiporter in Escherichia coli.”  Pinner E.et.al.   1317851
[7] “Two cysteines in each periplasmic domain of the membrane protein DsbB are required for its function in protein disulfide bond formation.”  Jander G.et.al.   7957076
[8] “Respiratory chain strongly oxidizes the CXXC motif of DsbB in the Escherichia coli disulfide bond formation pathway.”  Kobayashi T.et.al.   10064586
[9] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
Structure:
2HI7   2K73   2K74   2ZUP   2ZUQ   3E9J   2LEG   2LTQ     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MLRFLNQCSQ GRGAWLLMAF TALALELTAL WFQHVMLLKP CVLCIYERCA LFGVLGAALI 
61:	GAIAPKTPLR YVAMVIWLYS AFRGVQLTYE HTMLQLYPSP FATCDFMVRF PEWLPLDKWV 
121:	PQVFVASGDC AERQWDFLGL EMPQWLLGIF IAYLIVAVLV VISQPFKAKK RDLFGR