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5.B.1.5.2
Vacuolar Fe3+ and Cu2+ reductase, Fre6 (transfers electrons from NADPH in the cytoplasm to Cu2+ in the vacuole) (Rees and Thiele, 2007).

Accession Number:Q12473
Protein Name:Ferric reductase transmembrane component 6 aka Fre6
Length:712
Molecular Weight:81989.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:8
Location1 / Topology2 / Orientation3: Vacuole membrane1 / Multi-pass membrane protein2
Substrate Electrons

Cross database links:

Genevestigator: Q12473
eggNOG: fuNOG08331
HEGENOM: HBG202942
DIP: DIP-5019N
RefSeq: NP_013049.1   
Entrez Gene ID: 850675   
Pfam: PF08022    PF01794    PF08030   
KEGG: sce:YLL051C   

Gene Ontology

GO:0000324 C:fungal-type vacuole
GO:0016021 C:integral to membrane
GO:0005774 C:vacuolar membrane
GO:0009055 F:electron carrier activity
GO:0050660 F:FAD binding
GO:0000293 F:ferric-chelate reductase activity
GO:0005506 F:iron ion binding
GO:0015677 P:copper ion import
GO:0022900 P:electron transport chain
GO:0006880 P:intracellular sequestering of iron ion
GO:0006826 P:iron ion transport

References (8)

[1] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.”  Johnston M.et.al.   9169871
[2] “Metalloregulation of FRE1 and FRE2 homologs in Saccharomyces cerevisiae.”  Martins L.J.et.al.   9726978
[3] “Regulated expression of the Saccharomyces cerevisiae Fre1p/Fre2p Fe/Cu reductase related genes.”  Georgatsou E.et.al.   10341420
[4] “Global analysis of protein localization in budding yeast.”  Huh W.-K.et.al.   14562095
[5] “Direct activation of genes involved in intracellular iron use by the yeast iron-responsive transcription factor Aft2 without its paralog Aft1.”  Courel M.et.al.   16024809
[6] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
[7] “Identification of a vacuole-associated metalloreductase and its role in Ctr2-mediated intracellular copper mobilization.”  Rees E.M.et.al.   17553781
[8] “The metalloreductase Fre6p in Fe-efflux from the yeast vacuole.”  Singh A.et.al.   17681937

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MHRTLLFLTW LISLTKAFNI KLPHTEKKDH LESNAVLACA SYINTLKWSF DSSVVPGFYS 
61:	TICSYSPAFD TWSLCIFNSL TDQIIPMDNT SFEESLGNVR KTCSFVDKKF SNISLEQYYS 
121:	SLNNASSHAL EDYGSIESLS TSIRVDRETR SRWIRAFHAH AYNLDISSVY GAYLTYYFVI 
181:	VGIIAVFFHM SHYNGLNRAL FASRFVNYIR GHFVLPTFLV DKHANHFKFL NVEVFTGLMP 
241:	NSLEAWIIFG YTLANIIFLS ISYIIDPYNL IFNSHLSQFT RLLADRSGIL AFTQFPLIII 
301:	FTARNSFLEF LTGVKFNSFI SFHKWIGRIM VLNATIHSLS YSLFAIINHA FKISNKQLYW 
361:	KFGIASITVL CVLLVLSLGI VRKRHYEFFL YTHIILALLF FYCCWQHVKI FNGWKEWIVV 
421:	SLLIWGLEKL FRIWNILQFR FPKATLINLN TSNNPHDEMF KVIIPKYNRR WHSKPGQYCF 
481:	IYFLHPLVFW QCHPFTIIDE GEKCVLVIKP KSGLTRFIYN HILQSLNGKL QLRVAIEGPY 
541:	GPSNLHLDKF DHLLLLSGGT GLPGPLDHAI KLSRNPDKPK SIDLIMAIKN PSFLNGYKSE 
601:	ILELKNSRSH VNVQVYLTQK TAVTKAANAR DQLIHFDDIM TELTSFAHIG NARPNFSNVI 
661:	ENAIKSTPPG DSLAVVCCGP PVLVDDVRNT VSQKLLGYPE RIIEYFEEYQ CW