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8.A.1.1.1
Membrane Fusion Protein, EmrA, that function with MFS multidrug exporter, EmrB (Nishino and Yamaguchi 2001).

Accession Number:P27303
Protein Name:EmrA aka B2685
Length:390
Molecular Weight:42736.00
Species:Escherichia coli [83333]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Single-pass membrane protein2
Substrate NONE

Cross database links:

Genevestigator: P27303
EchoBASE: EB1329
EcoGene: EG11354
eggNOG: COG1566
HEGENOM: HBG624965
DIP: DIP-9502N
RefSeq: AP_003257.1    NP_417170.1   
Entrez Gene ID: 947166   
Pfam: PF00529   
BioCyc: EcoCyc:EG11354-MONOMER    ECOL168927:B2685-MONOMER   
KEGG: ecj:JW2660    eco:b2685   

Gene Ontology

GO:0009276 C:Gram-negative-bacterium-type cell wall
GO:0016021 C:integral to membrane
GO:0031226 C:intrinsic to plasma membrane
GO:0015238 F:drug transmembrane transporter activity
GO:0008565 F:protein transporter activity
GO:0006855 P:drug transmembrane transport
GO:0009306 P:protein secretion
GO:0046677 P:response to antibiotic

References (5)

[1] “Emr, an Escherichia coli locus for multidrug resistance.”  Lomovskaya O.et.al.   1409590
[2] “Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.”  Yamamoto Y.et.al.   9205837
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Multidrug resistance pumps in bacteria: variations on a theme.”  Lewis K.et.al.   8203018

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSANAETQTP QQPVKKSGKR KRLLLLLTLL FIIIAVAIGI YWFLVLRHFE ETDDAYVAGN 
61:	QIQIMSQVSG SVTKVWADNT DFVKEGDVLV TLDPTDARQA FEKAKTALAS SVRQTHQLMI 
121:	NSKQLQANIE VQKIALAKAQ SDYNRRVPLG NANLIGREEL QHARDAVTSA QAQLDVAIQQ 
181:	YNANQAMILG TKLEDQPAVQ QAATEVRNAW LALERTRIIS PMTGYVSRRA VQPGAQISPT 
241:	TPLMAVVPAT NMWVDANFKE TQIANMRIGQ PVTITTDIYG DDVKYTGKVV GLDMGTGSAF 
301:	SLLPAQNATG NWIKVVQRLP VRIELDQKQL EQYPLRIGLS TLVSVNTTNR DGQVLANKVR 
361:	STPVAVSTAR EISLAPVNKL IDDIVKANAG