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8.A.27.1.1
Alkylphosphocholine resistance/Ro-sensitive-3/Brefeldin-A sensitivity-3 protein, Lem3 (Hanson et al., 2003)

Accession Number:P42838
Protein Name:LEM3
Length:414
Molecular Weight:47438.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:2
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate NONE

Cross database links:

Genevestigator: P42838
eggNOG: fuNOG07491
HEGENOM: HBG525157
DIP: DIP-4598N
RefSeq: NP_014076.1   
Entrez Gene ID: 855393   
Pfam: PF03381   
KEGG: sce:YNL323W   

Gene Ontology

GO:0005783 C:endoplasmic reticulum
GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005515 F:protein binding
GO:0007166 P:cell surface receptor linked signaling pathway
GO:0045332 P:phospholipid translocation

References (8)

[1] “Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames.”  Maftahi M.et.al.   7645347
[2] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.”  Philippsen P.et.al.   9169873
[3] “A novel membrane protein, Ros3p, is required for phospholipid translocation across the plasma membrane in Saccharomyces cerevisiae.”  Kato U.et.al.   12133835
[4] “Lem3p is essential for the uptake and potency of alkylphosphocholine drugs, edelfosine and miltefosine.”  Hanson P.K.et.al.   12842877
[5] “Global analysis of protein expression in yeast.”  Ghaemmaghami S.et.al.   14562106
[6] “Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.”  Li X.et.al.   17330950
[7] “Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases.”  Smolka M.B.et.al.   17563356
[8] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MVNFDLGQVG EVFRRKDKGA IVSGDNPEEE EDVDASEFEE DEVKPVRTKN RRPKEDAFTQ 
61:	QRLAAINPVL TPRTVLPLYL LIAVVFVIVG GCILAQNSKV DEVTIYYQDC MTNATSSWSD 
121:	IPSEHWQFVF HKYKTYNTAP QWRFVDDESD DFTKQRGTCQ IRFTTPSDMK NNVYLNYVLE 
181:	KFAANHRRYV LSFSEDQIRG EDASYETVHD ATGINCKPLS KNADGKIYYP CGLIANSMFN 
241:	DTFPLQLTNV GDTSNNYSLT NKGINWESDK KRYKKTKYNY TQIAPPPYWE KMYPDGYNET 
301:	NIPDIQDWEE FQNWMRPGAF DKITKLIRIN KNDTLPAGEY QLDIGLHWPV LEFNGKKGIY 
361:	LTHGSHLGGR NPFLGIVYLI GGCICAAMAL ILLTFWLFGG RKIADASSLS WNMK