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8.A.27.1.4
The plant β-subunit ALIS1 (350aas; two probable TMSs) (functions with the α-subunit of TC#3.A.3.8.6) (Poulsen et al., 2008)

Accession Number:Q9LTW0
Protein Name:ALIS1
Length:350
Molecular Weight:39069.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:2
Location1 / Topology2 / Orientation3: Golgi apparatus membrane1 / Multi-pass membrane protein2
Substrate NONE

Cross database links:

Genevestigator: Q9LTW0
eggNOG: KOG2952
HEGENOM: HBG525157
RefSeq: NP_566435.1   
Entrez Gene ID: 820456   
Pfam: PF03381   
KEGG: ath:AT3G12740   

Gene Ontology

GO:0000139 C:Golgi membrane
GO:0016021 C:integral to membrane
GO:0015914 P:phospholipid transport

References (3)

[1] “Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones.”  Sato S.et.al.   10819329
[2] “Empirical analysis of transcriptional activity in the Arabidopsis genome.”  Yamada K.et.al.   14593172
[3] “The Arabidopsis P4-ATPase ALA3 localizes to the golgi and requires a beta-subunit to function in lipid translocation and secretory vesicle formation.”  Poulsen L.R.et.al.   18344284

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSSSNTPSSS AAAAGSIDSS AARRNSKRPK YSKFTQQELP ACKPILTPGW VISTFLIISV 
61:	IFIPLGVISL FASQDVVEIV DRYDSACIPL SDRANKVAYI QGTGNKSCTR TLIVPKRMKQ 
121:	PIYVYYQLEN FYQNHRRYVK SRSDSQLRSV KDENQIDACK PEDDFGGQPI VPCGLIAWSL 
181:	FNDTYVLSRN NQGLTVNKKG IAWKSDKEHK FGKNVFPKNF QKGNLTGGAS LDPNKPLSDQ 
241:	EDLIVWMRTA ALPTFRKLYG KIESDLEKGE NIQVTLQNNY NTYSFSGKKK LVLSTTSWLG 
301:	GKNDFLGIAY LTVGGICFVL ALAFTVMYLV KPRRLGDPTY LSWNRIPGGR