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8.A.3.3.1
Tyrosine protein kinase of 726 aas and 2 TMSs, Etk (MPA1-C).  Probably involved in the regulation of capsular polysaccharide export together with a tyrosine phosphatase, Etp of 148 aas (acc# P0ACZ2) (Peleg et al. 2005; Nadler et al. 2012).

Accession Number:P38134
Protein Name:Etk aka B0981
Length:726
Molecular Weight:81242.00
Species:Escherichia coli [83333]
Number of TMSs:2
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate NONE

Cross database links:

Genevestigator: P38134
EchoBASE: EB1773
EcoGene: EG11826
eggNOG: COG3206
HEGENOM: HBG309770
DIP: DIP-9526N
RefSeq: AP_001610.1    NP_415501.1   
Entrez Gene ID: 947409   
Pfam: PF02706   
BioCyc: EcoCyc:EG11826-MONOMER    ECOL168927:B0981-MONOMER    MetaCyc:EG11826-MONOMER   
KEGG: ecj:JW0964    eco:b0981   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005524 F:ATP binding
GO:0030234 F:enzyme regulator activity
GO:0004713 F:protein tyrosine kinase activity
GO:0045227 P:capsule polysaccharide biosynthetic process
GO:0009103 P:lipopolysaccharide biosynthetic process

References (7)

[1] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “The complete nucleotide sequence of the Escherichia coli gene appA reveals significant homology between pH 2.5 acid phosphatase and glucose-1-phosphatase.”  Dassa J.et.al.   2168385
[5] “Intrinsic and extrinsic approaches for detecting genes in a bacterial genome.”  Borodovsky M.et.al.   7984428
[6] “Relationship between exopolysaccharide production and protein-tyrosine phosphorylation in Gram-negative bacteria.”  Vincent C.et.al.   11090276
[7] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
Structure:
3CIO     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MTTKNMNTPP GSTQENEIDL LRLVGELWDH RKFIISVTAL FTLIAVAYSL LSTPIYQADT 
61:	LVQVEQKQGN AILSGLSDMI PNSSPESAPE IQLLQSRMIL GKTIAELNLR DIVEQKYFPI 
121:	VGRGWARLTK EKPGELAISW MHIPQLNGQD QQLTLTVGEN GHYTLEGEEF TVNGMVGQRL 
181:	EKDGVALTIA DIKAKPGTQF VLSQRTELEA INALQETFTV SERSKESGML ELTMTGDDPQ 
241:	LITRILNSIA NNYLQQNIAR QAAQDSQSLE FLQRQLPEVR SELDQAEEKL NVYRQQRDSV 
301:	DLNLEAKAVL EQIVNVDNQL NELTFREAEI SQLYKKDHPT YRALLEKRQT LEQERKRLNK 
361:	RVSAMPSTQQ EVLRLSRDVE AGRAVYLQLL NRQQELSISK SSAIGNVRII DPAVTQPQPV 
421:	KPKKALNVVL GFILGLFISV GAVLARAMLR RGVEAPEQLE EHGISVYATI PMSEWLDKRT 
481:	RLRKKNLFSN QQRHRTKNIP FLAVDNPADS AVEAVRALRT SLHFAMMETE NNILMITGAT 
541:	PDSGKTFVSS TLAAVIAQSD QKVLFIDADL RRGYSHNLFT VSNEHGLSEY LAGKDELNKV 
601:	IQHFGKGGFD VITRGQVPPN PSELLMRDRM RQLLEWANDH YDLVIVDTPP MLAVSDAAVV 
661:	GRSVGTSLLV ARFGLNTAKE VSLSMQRLEQ AGVNIKGAIL NGVIKRASTA YSYGYNYYGY 
721:	SYSEKE