TCDB is operated by the Saier Lab Bioinformatics Group

8.A.51 The Dipeptidyl-aminopeptidase-like Protein 6 beta subunit of Kv4 channels (DPP6) Family

Auxiliary beta-subunits dictate the physiological properties of voltage-gated K+ (KV) channels in excitable tissues. The dipeptidyl-aminopeptidase-like protein 6 (DPP6) is a specific beta-subunit of neuronal KV4 channels, which may promote gating through interactions between the single transmembrane segment of DPP6 and the channel's voltage sensing domain (VSD). A combination of gating current measurements and protein biochemistry (in-vitro translation and co- immunoprecipitations) revealed preferential physical interaction between the isolated KV4.2-VSD and DPP6 (Dougherty et al. 2009). Significantly weaker interactions were detected between DPP6 and KV1.3 channels or the KV4.2 pore domain. More efficient gating charge movement resulting from a direct interaction between DPP6 and the KV4.2-VSD is unique among the known actions of KV channel beta-subunits. Thus. the modular VSD of a KV channel can be directly regulated by transmembrane protein-protein interactions involving an extrinsic beta-subunit. Understanding these interactions may shed light on the pathophysiology of recently identified human disorders associated with mutations affecting the dpp6 gene.

References associated with 8.A.51 family:

Dougherty, K. and M. Covarrubias. (2006). A dipeptidyl aminopeptidase-like protein remodels gating charge dynamics in Kv4.2 channels. J Gen Physiol 128: 745-753. 17130523
Dougherty, K., L. Tu, C. Deutsch, and M. Covarrubias. (2009). The dipeptidyl-aminopeptidase-like protein 6 is an integral voltage sensor-interacting β-subunit of neuronal K(V)4.2 channels. Channels (Austin) 3: 122-128. 19372736

References associated with 8.a.51 family:

Fang, J. and Y. Wei. (2011). Expression, purification and characterization of the Escherichia coli integral membrane protein YajC. Protein Pept Lett 18: 601-608. 21235483
Gardel, C., K. Johnson, A. Jacq and J. Beckwith (1990). The secD locus of E. coli codes for two membrane proteins required for protein export. EMBO J. 9: 3209-3216. 2170107

References associated with 8.A.51 family:

Kuo, Y.L., J.K. Cheng, W.H. Hou, Y.C. Chang, P.H. Du, J.J. Jian, R.H. Rau, J.H. Yang, C.C. Lien, and M.L. Tsaur. (2017). K+ Channel Modulatory Subunits KChIP and DPP Participate in Kv4-Mediated Mechanical Pain Control. J. Neurosci. 37: 4391-4404. 28330877

References associated with 8.a.51 family:

Reuter, K., R. Slany, F. Ullrich and H. Kersten (1991). Structure and organization of Escherichia coli genes involved in biosynthesis of the deazaguanine derivative queuine, a nutrient factor for eukaryotes. J. Bacteriol. 173: 2256-2264. 1706703
Törnroth-Horsefield, S., P. Gourdon, R. Horsefield, L. Brive, N. Yamamoto, H. Mori, A. Snijder, and R. Neutze. (2007). Crystal structure of AcrB in complex with a single transmembrane subunit reveals another twist. Structure. 15: 1663-1673. 18073115